help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
This version published online on January 20, 2005
Endocrinology, doi:10.1210/en.2004-1431
A more recent version of this article appeared on May 1, 2005
This Article
Right arrow Author Manuscript (PDF)
Right arrow All Versions of this Article:
146/5/2229    most recent
Author Manuscript (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by PAE, H.-O.
Right arrow Articles by CHUNG, H.-T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by PAE, H.-O.
Right arrow Articles by CHUNG, H.-T.

Submitted on November 5, 2004
Accepted on January 12, 2005

A Molecular Cascade Showing Nitric Oxide-Heme Oxygenase-1-Vascular Endothelial Growth Factor-Interleukin-8 Sequence in Human Endothelial Cells

HYUN-OCK PAE, GI-SU OH, BYUNG-MIN CHOI, YOUNG-MYEONG KIM, and HUN-TAEG CHUNG*

Department of Microbiology and Immunology (H.O.P, G.S.O, B.M.C., H.T.C.), Wonkwang University School of Medicine, Iksan-Shi, Chonbug 570-749, Vascular System Research Center and Department of Molecular and Cellular Biochemistry (Y.M.K.), Kangwon National University School of Medicine, Chunchon, Kangwon-Do 200-701, Republic of Korea

* To whom correspondence should be addressed. E-mail: htchung{at}wonkwang.ac.kr.

Heme oxygenase (HO)-1 has been shown to be an important biological target of nitric oxide (NO). NO can induce HO-1 expression and interleukin (IL)-8 production, particularly, in endothelial cells. Interestingly, HO-1 tends to induce the production of vascular endothelial growth factor (VEGF) that is involved in endothelial IL-8 syntheses. Whether HO-1 expression by NO may provide a link with IL-8 or VEGF synthesis was investigated in human umbilical vein endothelial cells (HUVECs). The NO donor S-nitroso-N-acetyl-penicillamine (SNAP) dose-dependently increased IL-8 and VEGF productions and HO-1 expression in HUVECs. Transfection with either HO-1 small interfering RNA or HO-1 antisense oligodeoxynucleotide abrogated the ability of SNAP to induce HO-1 expression and IL-8 and VEGF productions. Both pharmacological induction and gene transfer of HO-1 directly induced IL-8 and VEGF productions. Anti-VEGF neutralizing antibody blocked SNAP-mediated IL-8 production and VEGF itself induced IL-8 production, whereas anti-IL-8 neutralizing antibody had no effect on VEGF production in SNAP-treated HUVECs. Neither anti-VEGF nor anti-IL-8 antibodies influenced SNAP-induced HO-1 expression. Moreover, neither VEGF nor IL-8 showed an additive effect on SNAP-induced HO-1 expression. HO-1 transfection had no significant effect on productions of other CXC chemokines, such as growth-related oncogen-{alpha} and epithelial neutrophil activation peptide-78. Taken together, these results provide a molecular cascade showing NO-HO-1-VEGF-IL-8 sequence in human endothelial cells.
Key words: Nitric oxide • Heme oxygenase-1 • Vascular endothelial growth factor • Interleukin-8 • Endothelial cells




This article has been cited by other articles:


Home page
 Cardiovasc ResHome page
J. Tongers, J.-M. Knapp, M. Korf, T. Kempf, A. Limbourg, F. P. Limbourg, Z. Li, D. Fraccarollo, J. Bauersachs, X. Han, et al.
Haeme oxygenase promotes progenitor cell mobilization, neovascularization, and functional recovery after critical hindlimb ischaemia in mice
Cardiovasc Res, May 1, 2008; 78(2): 294 - 300.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
J. Dulak, J. Deshane, A. Jozkowicz, and A. Agarwal
Heme Oxygenase-1 and Carbon Monoxide in Vascular Pathobiology: Focus on Angiogenesis
Circulation, January 15, 2008; 117(2): 231 - 241.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. Datta, O. Dormond, A. Basu, D. M. Briscoe, and S. Pal
Heme oxygenase-1 modulates the expression of the anti-angiogenic chemokine CXCL-10 in renal tubular epithelial cells
Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1222 - F1230.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
A. Steensberg, C. Keller, T. Hillig, C. Frosig, J. F. P. Wojtaszewski, B. K. Pedersen, H. Pilegaard, and M. Sander
Nitric oxide production is a proximal signaling event controlling exercise-induced mRNA expression in human skeletal muscle
FASEB J, September 1, 2007; 21(11): 2683 - 2694.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Abuarqoub, R. Foresti, C. J. Green, and R. Motterlini
Heme oxygenase-1 mediates the anti-inflammatory actions of 2'-hydroxychalcone in RAW 264.7 murine macrophages
Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1092 - C1099.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
N. M. Gharavi, N. A. Baker, K. P. Mouillesseaux, W. Yeung, H. M. Honda, X. Hsieh, M. Yeh, E. J. Smart, and J. A. Berliner
Role of Endothelial Nitric Oxide Synthase in the Regulation of SREBP Activation by Oxidized Phospholipids
Circ. Res., March 31, 2006; 98(6): 768 - 776.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2005 by The Endocrine Society