Involvement of Corticotropin-Releasing Hormone- and Interleukin (IL)-6-Dependent Proopiomelanocortin Induction in the Anterior Pituitary during Hypothalamic-Pituitary-Adrenal Axis Activation by IL-1

Supplemental Data

Supplemental Figures

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1: Induction of POMC transcript in the AP in response to peripheral injection of IL-1α in WT and IL-1α/β KO male mice. POMC mRNA levels in the AP were estimated by in situ hybridization after i.p. injection of rmIL-1α (20 µg/kg B.W) to WT and IL-1α/β KO mice. Numbers beneath each column show the number of animals per group. Levels relative to these of untreated mice (0 h) of WT mice were shown. a: Statistical significance (p< 0.05) between the other three groups determined by one-way ANOVA and Tukey’s test.
• Supplemental Figure 2 - Supplemental Figure 2: Figure S2. Induction of c-fos transcript in PVN in response to peripheral injection of IL-1α. (A) c-fos mRNA levels in the PVN were estimated by in situ hybridization after i.p. injection of rmIL-1α to WT mice. (B) c-fos mRNA levels in the PVN were measured after i.p. injection of rmIL-1α to IL-1α/β KO mice. Numbers beneath each column show the number of animals per group. Levels relative to these of untreated mice (0 h) of each genotype were shown. Statistical significance was determined by student’s t-test. #: p< 0.05.