help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McGowan, N. W. A.
Right arrow Articles by Helfrich, M. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McGowan, N. W. A.
Right arrow Articles by Helfrich, M. H.
Endocrinology Vol. 142, No. 4 1678
Copyright © 2001 by The Endocrine Society

ARTICLES

Cytokine-Activated Endothelium Recruits Osteoclast Precursors

Neil W. A. McGowan, Emily J. Walker, Heather Macpherson, Stuart H. Ralston and Miep H. Helfrich

Department of Medicine and Therapeutics, University of Aberdeen Medical School, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom

Address all correspondence and requests for reprints to: Miep H. Helfrich, University of Aberdeen Medical School, Polwarth Building, Department of Medicine and Therapeutics, Fosterhill AB25 2ZD, United Kingdom.


   Abstract
Top
 Abstract
Introduction
 
Osteoclast precursors reach sites of osteoclast formation and remodelling via the vasculature and are therefore destined to encounter endothelium before migrating to the bone surface. Here we investigated the hypothesis that endothelium may be involved in the regulation of osteoclast precursor recruitment to sites of bone resorption. Osteoclast precursors in human peripheral blood were identified by their ability to form mature osteoclasts in 21-day cultures supplemented with RANKLigand, M-CSF, 1,25(OH)2-vitamin D3, dexamethasone and prostaglandin E2. Under control conditions few osteoclast precursors adhered to endothelial cells (the human bone marrow-derived endothelial cell line BMEC-1). However, BMEC-1 cells treated with the resorption stimulating cytokines IL-1ß and TNF{alpha} depleted the PBMC population of all osteoclast precursors. These results provide the first evidence that osteoclast precursors can adhere to endothelium and suggest that endothelium could play an important role in the recruitment of osteoclast precursors to sites of bone resorption.


   Introduction
Top
 Abstract
 Introduction
 

Received December 11, 2001.




This article has been cited by other articles:


Home page
 Endocr. Rev.Home page
T. M. Doherty, L. A. Fitzpatrick, D. Inoue, J.-H. Qiao, M. C. Fishbein, R. C. Detrano, P. K. Shah, and T. B. Rajavashisth
Molecular, Endocrine, and Genetic Mechanisms of Arterial Calcification
Endocr. Rev., August 1, 2004; 25(4): 629 - 672.
[Abstract] [Full Text] [PDF]

Home page
 Crit. Rev. Oral Biol. Med.Home page
J.K. Hartsfield Jr., E.T. Everett, and R.A. Al-Qawasmi
GENETIC FACTORS IN EXTERNAL APICAL ROOT RESORPTION AND ORTHODONTIC TREATMENT
Crit. Rev. Oral. Biol. Med., March 1, 2004; 15(2): 115 - 122.
[Abstract] [Full Text]

Home page
 J. Clin. Endocrinol. Metab.Home page
N. W. A. McGowan, H. MacPherson, K. Janssens, W. Van Hul, J. C. Frith, W. D. Fraser, S. H. Ralston, and M. H. Helfrich
A Mutation Affecting the Latency-Associated Peptide of TGF{beta}1 in Camurati-Engelmann Disease Enhances Osteoclast Formation in Vitro
J. Clin. Endocrinol. Metab., July 1, 2003; 88(7): 3321 - 3326.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
T. M. DOHERTY, H. UZUI, L. A. FITZPATRICK, P. V. TRIPATHI, C. R. DUNSTAN, K. ASOTRA, and T. B. RAJAVASHISTH
Rationale for the role of osteoclast-like cells in arterial calcification
FASEB J, April 1, 2002; 16(6): 577 - 582.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McGowan, N. W. A.
Right arrow Articles by Helfrich, M. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McGowan, N. W. A.
Right arrow Articles by Helfrich, M. H.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals