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Regional Expression of mRNA Encoding a Second Form of Gonadotropin-Releasing Hormone in the Macaque Brain

Division of Neuroscience (H.F.U., S.G.K., V.T.G., V.S.D.); Oregon Regional Primate Research Center, Beaverton, Oregon 97006; Department of Physiology and Pharmacology (H.F.U.), Oregon Health Sciences University, Portland, Oregon 97201; and Program in Neuroscience (R.B.W., R.D.F.), Stanford University, Stanford, California 94305

Address all correspondence and requests for reprints to: Henryk F. Urbanski, Ph.D., Oregon Regional Primate Research Center, Division of Neuroscience, 505 NW 185th Avenue, Beaverton, Oregon 97006.

	Abstract

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In mammals, reproduction is thought to be controlled by a single neuropeptide, gonadotropin-releasing hormone (GnRH-I), which regulates the synthesis and secretion of gonadotropins from the pituitary gland. However, another form of this decapeptide (GnRH-II), of unknown function, also exists in the brain of many vertebrate species, including humans; it is encoded by a different gene and its amino acid sequence is 70% identical to that of GnRH-I. Here, we report the cloning of a GnRH-II cDNA from the rhesus macaque (Macaca mulatta), and show for the first time by in situ hybridization that GnRH-II mRNA is expressed in the primate midbrain, hippocampus and discrete nuclei of the hypothalamus, including the supraoptic, paraventricular, suprachiasmatic and arcuate. Because the regional distribution pattern of cells containing GnRH-II mRNA is largely dissimilar to that of cells containing GnRH-I mRNA, it is likely that these two cell populations receive distinct neuroendocrine inputs and thus regulate GnRH synthesis and release differently.

Received December 17, 1998.

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This Article Abstract Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Urbanski, H. F. Articles by Densmore, V. S. Search for Related Content PubMed PubMed Citation Articles by Urbanski, H. F. Articles by Densmore, V. S.