This Article Abstract Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Petroff, M. G. Articles by Pate, J. L. Search for Related Content PubMed PubMed Citation Articles by Petroff, M. G. Articles by Pate, J. L.

Expression of Cytokine Messenger Ribonucleic Acids in the Bovine Corpus Luteum

Department of Animal Sciences, Ohio State University, Wooster, Ohio 44691

Address all correspondence and requests for reprints to: Joy Lee Pate, OARDC, Department of Animal Science, 1680 Madison Avenue, Wooster, Ohio 44691.

	Abstract

Top Abstract

 
There is considerable evidence that luteolysis in the cow and other species involves components of the immune system. In this study, we examined the expression of the mRNAs for TNF-, IFN-, IL-1ß, II-2, and IL-2 receptor (IL-2R) by reverse transcription-polymerase chain reaction (RT-PCR) using bovine-specific primers. Expression was examined in corpora lutea (CL) of the early (day 5), mid (days 11–12), and late (day 18) luteal phase, and at 1, 4, and 24 hours following a luteolytic dose of prostaglandin (PG) F₂. Tumor necrosis factor-, IFN-, and IL-1ß mRNAs were detectable by RT-PCR at all stages of the cycle examined. Densitometric intensities of the electrophoresed IFN- PCR products revealed a drop in RNA expression during late diesstrus and at one hour of prostaglandin-induced luteolysis (P < 0.05). The mRNA for TNF- seemed to remain constant during the cycle, and rose slightly during luteolysis. Interleukin-1ß mRNA also did not vary during the cycle or during luteolysis. Finally, expression of mRNAs for IL-2 and IL-2 receptor was not evident in CL by the methods employed in this study. These results are the first to describe mRNA expression for TNF-, IFN-, and IL-1b in the bovine corpus luteum, and support a role for these cytokines in luteal function and regression.

Received October 19, 1998.

This Article Abstract Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Petroff, M. G. Articles by Pate, J. L. Search for Related Content PubMed PubMed Citation Articles by Petroff, M. G. Articles by Pate, J. L.