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Upregulation of Leptin Receptor Gene Expression in the Anterior Pituitary of Human Growth Hormone-Releasing Hormone Transgenic Mice

Department of Anatomy and Neurobiology, University of Kentucky College of Medicine, Lexington, Kentucky 40536

Address all correspondence and requests for reprints to: James F. Hyde, Ph.D., University of Kentucky Medical Center, Department of Anatomy & Neurobiology, 800 Rose Street (MN224), Lexington, Kentucky 40536-0084.

	Abstract

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The recently cloned leptin receptor (OB-R) is expressed in many tissues, including the anterior pituitary. It is not known whether OB-R gene expression is regulated by pituitary hormones. In the present study, we detected the long isoform of OB-R (OB-R_L) in the anterior pituitary of normal mice using RT-PCR, but were unable to detect the short isoform (OB-R_S). In human growth hormone-releasing hormone (hGHRH) transgenic mice, we discovered a significant increase in OB-R_L mRNA levels in the anterior pituitary as compared to controls, and OB-R_S gene expression was detectable. In contrast to the pituitary, there were no significant changes in OB-R gene expression for either isoform in the hypothalamus of hGHRH mice. The dramatic increase in the gene expression of OB-R_L in the anterior pituitary of hGHRH transgenic mice was confirmed by RNase protection assay. This is the first study to demonstrate that OB-R gene expression in the anterior pituitary gland is increased by GH and/or GHRH.

Received September 26, 1997.

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