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Expression of the Five Somatostatin Receptor (SSTR1-5) Subtypes in Rat Pituitary Somatotrophes: Quantitative Analysis by Double-Label Immunofluorescence Confocal Microscopy

Fraser Laboratories, Departments of Medicine and Neurology and Neurosurgery and Anatomy and Cell Biology (D.L.), McGill University, Royal Victoria Hospital and Montreal Neurological Institute, Montreal, Quebec, Canada; Department of Pharmacology (E.E.), University of Sherbrooke, Quebec, Canada

Address all correspondence and requests for reprints to: Yogesh C. Patel, M.D., Ph.D., McGill University, Department of Medicine, Neurology & Neurosurgery, Royal Victoria Hospital, Room M3-15, 687 Pine Avenue West, Montreal, Quebec H3A 1A1, Canada.

	Abstract

Top Abstract

 
Using quantitative double-label fluorescence immunocytochemistry and confocal microscopy, we have analysed the pattern of expression of SSTR1-5 in normal rat pituitary somatotrophes. Antipeptide rabbit polyclonal antibodies were produced against the extracellular domains of SSTR1-5. SSTR antigens were colocalized in GH positive cells using rhodamine conjugated secondary antibody for SSTRs and FITC-conjugated secondary antibody for GH. SSTR5 was the predominant subtype which was expressed in 86 ± 9.7% of GH cells followed by SSTR2 in 42 ± 6.4% of GH positive cells. SSTR4 and SSTR3 were modestly expressed in 23 ± 4.7% and 18 ± 3.2% of somatotrophes respectively whereas SSTR1 was the least expressed subtype occurring in only 5 ± 1.2% of somatotrophes. These results demonstrate variable expression of the 5 SSTRs in somatotrophes. The preponderance of the SST-28 preferring SSTR5 subtype correlates with the reported higher potency of SST-28 than SST-14 for inhibiting GH secretion.

Received July 9, 1997.

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