Endocrinology, Vol 99, 535-540, Copyright © 1976 by Endocrine Society

ARTICLES

Effect of anomers of D-glucose on alloxan inhibition of insulin release in isolated perifused pancreatic islets

ML McDaniel, CE Roth, CJ Fink and PE Lacy

The in vitro inhibition of insulin released by alloxan (20 mg/100 ml) in collagenase isolated rat islets is preferentially prevented by alpha D-glucose at a concentration of 1.0 mg/ml, while at a higher anomer concentration (1.5 mg/ml) both alpha and beta D-glucose provide equal protection. The ability of alpha D-glucose compared with beta D-glucose to stimulate insulin release, in vitro, showed a similar dose-related response, as observed in the alloxan protective studies. Although, both alpha and beta D-glucose compete with mutorated D-glucose for transport into islet cells, neither anomer produced a significantly different degree of inhibition in the transport process. The shared alpha stereospecificity for D-glucose in protection against alloxan and in stimulating insulin secretion in these in vitro studies, suggest a common site of interaction which may involve the beta-cell membrane.

This article has been cited by other articles:

				S. Woods and L. McKay Intraventricular alloxan eliminates feeding elicited by 2-deoxyglucose Science, December 15, 1978; 202(4373): 1209 - 1211. [Abstract] [PDF]