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Reduction of Insulin-Stimulated Glucose Uptake in L6 Myotubes by the Protein Kinase Inhibitor SB203580 Is Independent of p38MAPK Activity

Programme in Cell Biology (C.N.A., C.H., W.N., Z.L., P.J.B., A.K.), The Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8; Department of Biochemistry (C.N.A., A.K.) and Institute of Medical Sciences (C.H.), University of Toronto, Ontario, Canada M5S 1A8; School of Life Sciences (P.A.E.), University of Manchester, Manchester M13 9PT, United Kingdom; and Department of Pharmacology (K.A.H.), University of Colorado Health Sciences Center and Denver Veterans Affairs Medical Center, Denver, Colorado 80262

Address all correspondence and requests for reprints to: A. Klip, Programme in Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8. E-mail: amira{at}sickkids.ca.

Insulin increases glucose uptake through translocation of the glucose transporter GLUT4 to the plasma membrane. We previously showed that insulin activates p38MAPK, and inhibitors of p38MAPK and p38MAPKß (e.g. SB203580) reduce insulin-stimulated glucose uptake without affecting GLUT4 translocation. This observation suggested that insulin may increase GLUT4 activity via p38 and/or p38ß. Here we further explore the possible participation of p38MAPK through a combination of molecular strategies. SB203580 reduced insulin stimulation of glucose uptake in L6 myotubes overexpressing an SB203580-resistant p38 (drug-resistant p38) but barely affected phosphorylation of the p38 substrate MAPK-activated protein kinase-2. Expression of dominant-negative p38 or p38ß reduced p38MAPK phosphorylation by 70% but had no effect on insulin-stimulated glucose uptake. Gene silencing via isoform-specific small interfering RNAs reduced expression of p38 or p38ß by 60–70% without diminishing insulin-stimulated glucose uptake. SB203580 reduced photoaffinity labeling of GLUT4 by bio-LC-ATB-BMPA only in the insulin-stimulated state. Unless low levels of p38MAPK suffice to regulate glucose uptake, these results suggest that the inhibition of insulin-stimulated glucose transport by SB203580 is likely not mediated by p38MAPK. Instead, changes experienced by insulin-stimulated GLUT4 make it susceptible to inhibition by SB203580.

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