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Identification of a Glycogenolysis-Inhibiting Peptide from the Corpora Cardiaca of Locusts

Laboratory for Developmental Physiology and Molecular Biology (E.C., J.H., G.B., A.D.L., L.S.), Katholieke Universiteit Leuven, 3000 Leuven, Belgium; and Department of Biochemical Physiology (J.V.D., D.V.d.H.), Utrecht University, 3584 CH Utrecht, The Netherlands

Address all correspondence and requests for reprints to: Elke Clynen, Zoological Institute, Naamsestraat 59, B-3000 Leuven, Belgium. E-mail: Elke.Clynen{at}bio.kuleuven.ac.be.

A mass spectrometric study of the peptidome of the neurohemal part of the corpora cardiaca of Locusta migratoria and Schistocerca gregaria shows that it contains several unknown peptides. We were able to identify the sequence of one of these peptides as pQSDLFLLSPK. This sequence is identical to the part of the Locusta insulin-related peptide (IRP) precursor that is situated between the signal peptide and the B-chain. We designated this peptide as IRP copeptide. This IRP copeptide is also present in the pars intercerebralis, which is likely to be the site of synthesis. It is identical in both L. migratoria and S. gregaria. It shows no effect on the hemolymph lipid concentration in vivo or muscle contraction in vitro. The IRP copeptide is able to cause a decreased phosphorylase activity in locust fat body in vitro, opposite to the effect of the adipokinetic hormones and therefore possibly represents a glycogenolysis-inhibiting peptide.

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