help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kajita, K.
Right arrow Articles by Yasuda, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kajita, K.
Right arrow Articles by Yasuda, K.
Endocrinology Vol. 144, No. 1 253-259
Copyright © 2003 by The Endocrine Society

ARTICLE

Dehydroepiandrosterone Down-Regulates the Expression of Peroxisome Proliferator-Activated Receptor {gamma} in Adipocytes

Kazuo Kajita, Tatsuo Ishizuka, Tomoatsu Mune, Atsushi Miura, Masayoshi Ishizawa, Yoshinori Kanoh, Yasunori Kawai, Yoshiyuki Natsume and Keigo Yasuda

The Third Department of Internal Medicine (K.K., T.M., A.M., M.I., Y.Kan., Y.Kaw., Y.N., K.Y.) and Department of General Medicine (T.I.), Gifu University School of Medicine, Tsukasa-machi 40, Gifu 500-8705, Japan

Address all correspondence and requests for reprints to: Tatsuo Ishizuka, Department of General Medicine, Gifu University School of Medicine, Tsukasa-Machi 40, Gifu 500-8705, Japan. E-mail: tishizuk-gif{at}umin.ac.jp.

Dehydroepiandrosterone (DHEA) is expected to have a weight-reducing effect. In this study, we evaluated the effect of DHEA on genetically obese Otsuka Long Evans Fatty rats (OLETF) compared with Long-Evans Tokushima rats (LETO) as control. Feeding with 0.4% DHEA-containing food for 2 wk reduced the weight of sc, epididymal, and perirenal adipose tissue in association with decreased plasma leptin levels in OLETF. Adipose tissue from OLETF showed increased expression of peroxisome proliferator-activated receptor {gamma} (PPAR{gamma}) protein, which was prevented by DHEA treatment. Further, we examined the effect of DHEA on PPAR{gamma} in primary cultured adipocytes and monolayer adipocytes differentiated from rat preadipocytes. PPAR{gamma} protein level was decreased in a time- and concentration-dependent manner, and DHEA significantly reduced mRNA levels of PPAR{gamma}, adipocyte lipid-binding protein, and sterol regulatory element-binding protein, but not CCAAT/enhancer binding protein {alpha}. DHEA-sulfate also reduced the PPAR{gamma} protein, but dexamethasone, testosterone, or androstenedione did not alter its expression. In addition, treatment with DHEA for 5 d reduced the triglyceride content in monolayer adipocytes. These results suggest that DHEA down-regulates adiposity through the reduction of PPAR{gamma} in adipocytes.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
M. Aragno, R. Mastrocola, C. Medana, F. Restivo, M. G. Catalano, N. Pons, O. Danni, and G. Boccuzzi
Up-Regulation of Advanced Glycated Products Receptors in the Brain of Diabetic Rats Is Prevented by Antioxidant Treatment
Endocrinology, December 1, 2005; 146(12): 5561 - 5567.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
Y. Gosmain, N. Dif, V. Berbe, E. Loizon, J. Rieusset, H. Vidal, and E. Lefai
Regulation of SREBP-1 expression and transcriptional action on HKII and FAS genes during fasting and refeeding in rat tissues
J. Lipid Res., April 1, 2005; 46(4): 697 - 705.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
K. Dhatariya, M. L. Bigelow, and K. S. Nair
Effect of Dehydroepiandrosterone Replacement on Insulin Sensitivity and Lipids in Hypoadrenal Women
Diabetes, March 1, 2005; 54(3): 765 - 769.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2003 by The Endocrine Society