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Prenatal LHRH Neurons in Nasal Explant Cultures Express Estrogen Receptor ß Transcript

Cellular and Developmental Neurobiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892

Address all correspondence and requests for reprints to: Susan Wray, Chief, Cellular and Developmental Neurobiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Building 36, Room 5A-21, Bethesda, Maryland 20892-4156. E-mail: . swray{at}codon.nih.gov

Sex steroids influence LHRH neuronal activity, exerting a negative or positive feedback action, depending on the reproductive state of the animal. Recent evidence indicates that LHRH neurons possess the estrogen receptor ß (ERß) subtype postnatally, suggesting that estrogen may act, in part, directly on LHRH neurons. In this study, we identified ERß transcript in prenatal LHRH neurons as a function of age. Single-cell cDNA pools were made from LHRH neurons maintained for 7, 14, and 28 d in vitro (div). Screening of the cDNA pools by PCR with ERß-specific primers revealed ERß-positive LHRH neurons at all three ages. However, the number of LHRH cells coexpressing ERß transcript decreased dramatically between 14 (6/10) and 28 div (1/10). None of the LHRH cells were positive for ER transcript. These results indicate that developing LHRH neurons express the transcript for ERß and suggest that continued expression of ERß is either a characteristic of LHRH neurons that may require cues from the central nervous system and/or periphery or predetermined to be maintained in a subpopulation of LHRH neurons.

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