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IGF-I Inhibits Spontaneous Apoptosis in Human Granulocytes

Department of Pharmacology, Medical School, Vrije Universiteit Brussel, B-1090 Brussels, Belgium

Address all correspondence and requests for reprints to: Ron Kooijman, Department of Pharmacology, Medical School, Free University of Brussels (VUB), Laarbeeklaan 103, B-1090 Brussels, Belgium. E-mail: . rkooi{at}farc.vub.ac.be

Granulocytes are key cells in inflammatory processes that are recruited to sites of inflammation by chemoattractants such as IL-8 produced by neutrophils and monocytes. Programmed cell death (apoptosis) of granulocytes and subsequent recognition and phagocytosis by macrophages is a crucial mechanism for resolution of inflammation. Because IGF-I is a potent antiapoptotic factor, we addressed the effects of IGF-I on in vitro apoptosis of human peripheral blood granulocytes. We detected 1390 ± 467 IGF-I receptors with a dissociation constant of 2.3 ± 0.9 nM on purified granulocytes. Using microscopical analysis, annexin V binding assays to detect relocation of phosphatidylserine to the cell surface, and DNA fragmentation assays, we showed that IGF-I inhibits spontaneous apoptosis of granulocytes in serum-free culture by 32–45%. IGF-I did not modulate the secretion of IL-6, TNF, and IL-8 by granulocytes, but IL-8 secretion by peripheral blood mononuclear cells was enhanced by 40%. These observations indicate that IGF-I may promote granulocyte functions by increasing granulocyte longevity.

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