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Université de Rennes I, Interactions Cellulaires et Moléculaires, Equipe Information et Programmation Cellulaires, Centre National de la Recherche Scientifique, UMR 6026, Campus de Beaulieu, 35042 Rennes, France
Address all correspondence and requests for reprints to: Dr. M.-L. Thieulant, Equipe Information et Programation Cellulaires, UMR 6026, Bat 13, Campus de Beaulieu, 35042 Rennes Cedex, France. E-mail: marie-lise.thieulant{at}univ-rennes1.fr.
The aim of this study was to examine whether the expression levels of mRNA of the three estrogen receptor (ER) subtypes, ER
, ERß, and truncated ER product-1 (TERP-1) found in the rat pituitary gland were modified during gestation, lactation, and postlactation periods. By using relative quantitative RT-PCR, we found that ER mRNA significantly peaked in midpregnancy. However, the ER protein level remained constant. ERß gene expression did not change throughout pregnancy, suggesting that it was not related to estradiol levels during this reproductive period. In contrast, both TERP-1 mRNA and protein levels dramatically increased throughout the second half of gestation, being faintly detectable in early pregnancy. TERP-1 expression was rapidly reversed by lactation, whereas neither pituitary ER nor ERß relative levels were significantly altered. In addition, pup removal for 24–96 h on d 9 postpartum significantly reduced the expression of both ER and ERß mRNA compared with that in lactating animals, but the expression of TERP-1 mRNA was no longer detected. Collectively, our data indicate that 1) TERP-1, ER, and ERß expression levels are differentially regulated in the pituitary; 2) TERP-1 is variably expressed depending on the hormonal environment related to the estrous cycle, pregnancy, and lactation; and 3) TERP-1/ER ratios dramatically change depending on reproductive periods, suggesting a critical role for TERP-1 in reproductive events.
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