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Divergent Immune Responses in Male and Female Mice after Trauma-Hemorrhage: Dimorphic Alterations in T Lymphocyte Steroidogenic Enzyme Activities

Center for Surgical Research and Department of Surgery, University of Alabama, Birmingham, Alabama 35294

Address all correspondence and requests for reprints to: Irshad H. Chaudry, Ph.D., Center for Surgical Research, Department of Surgery, University of Alabama, Volker Hall G094, 1670 University Boulevard, Birmingham, Alabama 35294. E-mail: irshad.chaudry{at}ccc.uab.edu

Immune responses are suppressed in males, but not in proestrous females, after trauma-hemorrhage. Testosterone and 17ß-estradiol appear to be responsible for divergent immune effects. There is considerable evidence to suggest sex steroid hormone involvement in immune functions. As formation of active steroid depends on the activity of androgen- and estrogen-synthesizing enzymes, expression and activity of 5-reductase, aromatase, and 3ß- and 17ß- hydroxysteroid dehydrogenases were determined in spleen and T lymphocytes of male and proestrous female mice after trauma-hemorrhage. All of the enzymes were present in spleen, specifically in T lymphocytes. 5-Reductase expression and activity increased in male T lymphocytes, whereas aromatase activity, but not expression, increased in female T lymphocytes. Increased 5-reductase activity in male T lymphocytes is immunosuppressive because of increased 5-dihydrotestosterone synthesis, whereas in females increased aromatase activity triggering 17ß-estradiol synthesis is immunoprotective. This study also demonstrates the importance of 17ß-hydroxysteroid dehydrogenase oxidative and reductive functions. The immunoprotection of proestrous females is associated with enhanced reductase function of the enzyme. In males, decreased expression of oxidative isomer type IV, which impairs catabolism of 5-dihydrotestosterone, probably augments immunosuppression. This study provides evidence for the involvement of intracrine sex steroid synthesis in gender dimorphic immune responses after trauma-hemorrhage.