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Cloning of a Novel Growth Hormone-Regulated Rat Complementary Deoxyribonucleic Acid with Homology to the Human 1B-Glycoprotein, Characterizing a New Protein Family¹

Department of Medical Nutrition (C.G., A.M.), Karolinska Institutet, Novum, S-14186 Huddinge, Sweden; and Stockholm Bioinformatics Centre and Department of Biochemistry and Biophysics (B.P.), Karolinska Institutet, S-171 77 Stockholm, Sweden

Address all correspondence and requests for reprints to: Agneta Mode, Ph.D., Department of Medical Nutrition, Karolinska Institutet, Novum, S-14186 Huddinge, Sweden. E-mail: agneta.mode{at}mednut.ki.se

A sex-specific secretion of GH prevails in the rat. This has bearings on the expression of target genes, particularly in the liver. We have used suppressive subtractive hybridization to search for genes expressed in response to the female-characteristic, near-continuous secretion of GH. One sequence was particularly abundant among the obtained clones. After isolation of the corresponding full-length complementary DNA using rapid amplification of complementary DNA ends, it was found to be homologous to the human 1B-glycoprotein. Sequence comparisons suggest that the human 1B-glycoprotein and the rat homolog are members of a new family of proteins, of which at least four additional forms were found in the databases of human and mouse expressed sequence tags. In situ hybridization confirmed the female-specific expression, and by RNase protection analysis a liver-specific expression was indicated. Up-regulation of the messenger RNA by continuous exposure to GH, but not to the male-characteristic intermittent exposure, was demonstrated in hypophysectomized rats and in cultured primary hepatocytes.