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Mrp3, a Mitogen-Regulated Protein/Proliferin Gene Expressed in Wound Healing and in Hair Follicles¹

Molecular, Cellular and Developmental Biology Program (J.T.F., M.N.-H.) and the Departments of Zoology and Genetics (J.T.F.) and Biochemistry, Biophysics, and Molecular Biology (M.N.-H.), Iowa State University, Ames, Iowa 50011

Address all correspondence and requests for reprints to: Dr. Marit Nilsen-Hamilton, Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, 3206 Molecular Biology Building, Ames, Iowa 50011-3260. E-mail: marit{at}iastate.edu

During cutaneous wound healing, a marked increase in the local expression of growth factors results in increased migration and proliferation of the cells responsible for tissue repair. The mitogen-regulated protein (MRP)/proliferin proteins are growth factors and angiogenesis factors. Here it is demonstrated that Mrp3 is induced in wound edge keratinocytes during cutaneous wound healing and also temporally appears in the outer root sheath of the hair follicle during the late anagen phase of the hair cycle. In cultured keratinocytes, Mrp3 is induced by keratinocyte growth factor, but not by epidermal growth factor or by transforming growth factor type . Transgenic mice, expressing lacZ under the combined control of the cytomegalovirus immediate early enhancer and the Mrp3 flanking sequences, demonstrate wound- and hair cycle-induced transgene expression. These results show that elements within the flanking regulatory sequences of the Mrp3 gene are involved in the activation of Mrp3 in response to these events. The results reported here suggest that MRP3 may participate in wound healing and hair follicle cycle as a growth factor and/or angiogenesis factor.