help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Song, K.-H.
Right arrow Articles by Choi, H.-S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Song, K.-H.
Right arrow Articles by Choi, H.-S.
Endocrinology Vol. 142, No. 12 5116-5123
Copyright © 2001 by The Endocrine Society

RECEPTORS

LH Induces Orphan Nuclear Receptor Nur77 Gene Expression in Testicular Leydig Cells

Kwang-Hoon Song, Jae-Il Park, Mi-Ock Lee, Jaemog Soh, Keesook Lee and Hueng-Sik Choi

Hormone Research Center, Chonnam National University (K.-H.S., J.-I.P., J.S., K.L., H.-S.C.), Kwangju 500-757, and Department of Bioscience and Biotechnology, Sejong University (M.-O.L.), Seoul 143-747, Republic of Korea

Address all correspondence and requests for reprints to: Hueng-Sik Choi, Ph.D., Hormone Research Center, Chonnam National University, Kwangju 500-757, Republic of Korea. E-mail: hsc{at}chonnam.ac.kr

The orphan nuclear receptor Nur77 (NR4A1) is a member of the nuclear receptor superfamily and plays an important role in the regulation of genes involved in steroidogenesis and cell death. Northern blot analysis revealed that the expression of Nur77 mRNA was increased after puberty in mouse testis, and hCG treatment of peripubertal animals induced this gene expression in the testis. Moreover, LH treatment induced a transient increase in Nur77 mRNA, and this induction was LH dose dependent in mouse Leydig tumor cell line, K28. Western blot analysis showed that LH transiently induced Nur77 protein. The protein kinase inhibitor H-89, bisindolymaleimide I, and wortmannin strongly inhibited this inductive effect of LH on Nur77 gene expression. Transient transfection assay demonstrated that LH significantly increased the Nur77 promoter-driven luciferase reporter activity in a dose-dependent manner, and LH also increased the activity of a luciferase reporter gene driven by a promoter containing multi copies of a Nur77-responsive element. Moreover, EMSA showed that Nur77 DNA-binding activity was increased in response to LH. Finally, overexpression of dominant negative Nur77 reduced LH-mediated progesterone biosynthesis. Taken together, these results demonstrate that LH induces Nur77 gene expression, and Nur77 may play an important role in the LH-mediated steroidogenesis in Leydig cells.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
P. R. Manna, M. T. Dyson, Y. Jo, and D. M. Stocco
Role of Dosage-Sensitive Sex Reversal, Adrenal Hypoplasia Congenita, Critical Region on the X Chromosome, Gene 1 in Protein Kinase A- and Protein Kinase C-Mediated Regulation of the Steroidogenic Acute Regulatory Protein Expression in Mouse Leydig Tumor Cells: Mechanism of Action
Endocrinology, January 1, 2009; 150(1): 187 - 199.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
L. J Martin and J. J Tremblay
Glucocorticoids antagonize cAMP-induced Star transcription in Leydig cells through the orphan nuclear receptor NR4A1
J. Mol. Endocrinol., September 1, 2008; 41(3): 165 - 175.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
L. J. Martin, N. Boucher, C. Brousseau, and J. J. Tremblay
The Orphan Nuclear Receptor NUR77 Regulates Hormone-Induced StAR Transcription in Leydig Cells through Cooperation with Ca2+/Calmodulin-Dependent Protein Kinase I
Mol. Endocrinol., September 1, 2008; 22(9): 2021 - 2037.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
Y. D. Kim, K.-G. Park, Y.-S. Lee, Y.-Y. Park, D.-K. Kim, B. Nedumaran, W. G. Jang, W.-J. Cho, J. Ha, I.-K. Lee, et al.
Metformin Inhibits Hepatic Gluconeogenesis Through AMP-Activated Protein Kinase-Dependent Regulation of the Orphan Nuclear Receptor SHP
Diabetes, February 1, 2008; 57(2): 306 - 314.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. Tamura, M. Furihata, T. Tsunoda, S. Ashida, R. Takata, W. Obara, H. Yoshioka, Y. Daigo, Y. Nasu, H. Kumon, et al.
Molecular Features of Hormone-Refractory Prostate Cancer Cells by Genome-Wide Gene Expression Profiles
Cancer Res., June 1, 2007; 67(11): 5117 - 5125.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B. Liu, J.-f. Wu, Y.-y. Zhan, H.-z. Chen, X.-y. Zhang, and Q. Wu
Regulation of the Orphan Receptor TR3 Nuclear Functions by c-Jun N Terminal Kinase Phosphorylation
Endocrinology, January 1, 2007; 148(1): 34 - 44.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K.-H. Song, Y.-Y. Park, H. J. Kee, C. Y. Hong, Y.-S. Lee, S.-W. Ahn, H.-J. Kim, K. Lee, H. Kook, I.-K. Lee, et al.
Orphan Nuclear Receptor Nur77 Induces Zinc Finger Protein GIOT-1 Gene Expression, and GIOT-1 Acts as a Novel Corepressor of Orphan Nuclear Receptor SF-1 via Recruitment of HDAC2
J. Biol. Chem., June 9, 2006; 281(23): 15605 - 15614.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
N. M. Robert, L. J. Martin, and J. J. Tremblay
The Orphan Nuclear Receptor NR4A1 Regulates Insulin-Like 3 Gene Transcription in Leydig Cells
Biol Reprod, February 1, 2006; 74(2): 322 - 330.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
L. J. Martin, H. Taniguchi, N. M. Robert, J. Simard, J. J. Tremblay, and R. S. Viger
GATA Factors and the Nuclear Receptors, Steroidogenic Factor 1/Liver Receptor Homolog 1, Are Key Mutual Partners in the Regulation of the Human 3{beta}-Hydroxysteroid Dehydrogenase Type 2 Promoter
Mol. Endocrinol., September 1, 2005; 19(9): 2358 - 2370.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
X. Luo, L. Ding, J. Xu, R. S. Williams, and N. Chegini
Leiomyoma and Myometrial Gene Expression Profiles and Their Responses to Gonadotropin-Releasing Hormone Analog Therapy
Endocrinology, March 1, 2005; 146(3): 1074 - 1096.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. J. Martin and J. J. Tremblay
The Human 3{beta}-Hydroxysteroid Dehydrogenase/{Delta}5-{Delta}4 Isomerase Type 2 Promoter Is a Novel Target for the Immediate Early Orphan Nuclear Receptor Nur77 in Steroidogenic Cells
Endocrinology, February 1, 2005; 146(2): 861 - 869.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
J. C. Havelock, A. L. Smith, J. B. Seely, C. A. Dooley, R. J. Rodgers, W. E. Rainey, and B. R. Carr
The NGFI-B family of transcription factors regulates expression of 3{beta}-hydroxysteroid dehydrogenase type 2 in the human ovary
Mol. Hum. Reprod., February 1, 2005; 11(2): 79 - 85.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-G. Yoo, M. G. Yeo, D. K. Kim, H. Park, and M.-O. Lee
Novel Function of Orphan Nuclear Receptor Nur77 in Stabilizing Hypoxia-inducible Factor-1{alpha}
J. Biol. Chem., December 17, 2004; 279(51): 53365 - 53373.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
W. Li, H. Amri, H. Huang, C. Wu, and V. Papadopoulos
Gene and Protein Profiling of the Response of MA-10 Leydig Tumor Cells to Human Chorionic Gonadotropin
J Androl, November 1, 2004; 25(6): 900 - 913.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
K.-H. Song, Y.-Y. Park, K. C. Park, C. Y. Hong, J. H. Park, M. Shong, K. Lee, and H.-S. Choi
The Atypical Orphan Nuclear Receptor DAX-1 Interacts with Orphan Nuclear Receptor Nur77 and Represses Its Transactivation
Mol. Endocrinol., August 1, 2004; 18(8): 1929 - 1940.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
H.-J. Shin, B.-H. Lee, M. G. Yeo, S.-H. Oh, J.-D. Park, K.-K. Park, J.-H. Chung, C.-K. Moon, and M.-O. Lee
Induction of orphan nuclear receptor Nur77 gene expression and its role in cadmium-induced apoptosis in lung
Carcinogenesis, August 1, 2004; 25(8): 1467 - 1475.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Y. Hong, J. H. Park, R. S. Ahn, S. Y. Im, H.-S. Choi, J. Soh, S. H. Mellon, and K. Lee
Molecular Mechanism of Suppression of Testicular Steroidogenesis by Proinflammatory Cytokine Tumor Necrosis Factor Alpha
Mol. Cell. Biol., April 1, 2004; 24(7): 2593 - 2604.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. J. Tremblay and R. S. Viger
Transcription Factor GATA-4 Is Activated by Phosphorylation of Serine 261 via the cAMP/Protein Kinase A Signaling Pathway in Gonadal Cells
J. Biol. Chem., June 6, 2003; 278(24): 22128 - 22135.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
D. J. McLean, P. J. Friel, D. Pouchnik, and M. D. Griswold
Oligonucleotide Microarray Analysis of Gene Expression in Follicle-Stimulating Hormone-Treated Rat Sertoli Cells
Mol. Endocrinol., December 1, 2002; 16(12): 2780 - 2792.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K.-H. Song, K. Lee, and H.-S. Choi
Endocrine Disrupter Bisphenol A Induces Orphan Nuclear Receptor Nur77 Gene Expression and Steroidogenesis in Mouse Testicular Leydig Cells
Endocrinology, June 1, 2002; 143(6): 2208 - 2215.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2001 by The Endocrine Society