help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fernandez, P. M.
Right arrow Articles by Zakin, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fernandez, P. M.
Right arrow Articles by Zakin, M. M.
Endocrinology Vol. 141, No. 7 2392-2400
Copyright © 2000 by The Endocrine Society

ARTICLES

Nuclear Receptors Nor1 and NGFI-B/Nur77 Play Similar, Albeit Distinct, Roles in the Hypothalamo-Pituitary-Adrenal Axis1

Pablo M. Fernandez2, Franck Brunel, María Arroyo Jimenez, José M. Saez, Silvia Cereghini and Mario M. Zakin

Unité d’Expression des Gènes Eucaryotes (P.M.F., F.B., M.M.Z.) and Unité de Neurobiologie Moleculaire (M.A.J.), Institut Pasteur, 75724 Paris; INSERM, U-369, Faculté de Médecine Laennec (J.M.S.), 69372 Lyon; and INSERM, U-423, Hôpital Necker (S.C.), 75015 Paris, France

Address all correspondence and requests for reprints to: Dr. Mario M. Zakin, Unité d’Expression des Gènes Eucaryotes, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France. E-mail: mzakin{at}pasteur.fr

Studies in Nur77-deficient mice have shown that the basal regulation of hypothalamic and pituitary functions as well as the adrenocortical steroidogenesis in these animals is normal. This indicates that Nur77-related orphan receptors may substitute Nur77 functions in the hypothalamo-pituitary-adrenal axis by a compensatory mechanism. Nor1 is the most recently cloned member of the NGFI-B/Nur77 subfamily, and its properties are still largely unknown. We demonstrate here that Nor1 is expressed in the pituitary gland and adrenal cortex, and that ACTH and angiotensin II (AngII) treatment of adrenal fasciculata cells induces Nor1 expression. Time-course analysis with both hormones on steroidogenic capacity and the specific gene expression in adrenal cells strongly suggest that Nor1 is an intermediate in the long-term consequences of ACTH or AngII treatment. The Nor1 and NGFI-B/Nur77 amino acid sequence homology and the analysis of the trans-activation properties of Nor1 show that the overall structural and functional organization of the two proteins is similar. As observed with NGFI-B/Nur77, Nor1 activates the expression of genes encoding steroidogenic enzymes as P450c21, through its interaction with NGFI-B response element promoter sequences. In contrast, binding experiments of Nor1 with the palindromic NurRE sequence suggest that Nor1 is not an efficient substitute for the NGFI-B/Nur77 activation of the POMC gene expression in pituitary glands. All these results indicate that Nor1 and NGFI-B/Nur77 may play similar albeit distinct roles in the hypothalamo-pituitary-adrenal axis. Further experiments also show that the mechanisms responsible for the transcriptional regulation of Nor1 in adrenal cells appear to depend on the protein kinase A and protein kinase C cascades.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
M. A. Pearen, S. A. Myers, S. Raichur, J. G. Ryall, G. S. Lynch, and G. E. O. Muscat
The Orphan Nuclear Receptor, NOR-1, a Target of {beta}-Adrenergic Signaling, Regulates Gene Expression that Controls Oxidative Metabolism in Skeletal Muscle
Endocrinology, June 1, 2008; 149(6): 2853 - 2865.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
E. F Nogueira, C. A Vargas, M. Otis, N. Gallo-Payet, W. B Bollag, and W. E Rainey
Angiotensin-II acute regulation of rapid response genes in human, bovine, and rat adrenocortical cells
J. Mol. Endocrinol., December 1, 2007; 39(6): 365 - 374.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
D. G. Romero, S. Rilli, M. W. Plonczynski, L. L. Yanes, M. Y. Zhou, E. P. Gomez-Sanchez, and C. E. Gomez-Sanchez
Adrenal transcription regulatory genes modulated by angiotensin II and their role in steroidogenesis
Physiol Genomics, June 19, 2007; 30(1): 26 - 34.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. G. Smith and G. E. O. Muscat
Orphan nuclear receptors: therapeutic opportunities in skeletal muscle
Am J Physiol Cell Physiol, August 1, 2006; 291(2): C203 - C217.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
X. Luo, L. Ding, J. Xu, R. S. Williams, and N. Chegini
Leiomyoma and Myometrial Gene Expression Profiles and Their Responses to Gonadotropin-Releasing Hormone Analog Therapy
Endocrinology, March 1, 2005; 146(3): 1074 - 1096.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
J. C. Havelock, A. L. Smith, J. B. Seely, C. A. Dooley, R. J. Rodgers, W. E. Rainey, and B. R. Carr
The NGFI-B family of transcription factors regulates expression of 3{beta}-hydroxysteroid dehydrogenase type 2 in the human ovary
Mol. Hum. Reprod., February 1, 2005; 11(2): 79 - 85.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
N. Ke, G. Claassen, D.-H. Yu, A. Albers, W. Fan, P. Tan, M. Grifman, X. Hu, K. DeFife, V. Nguy, et al.
Nuclear Hormone Receptor NR4A2 Is Involved in Cell Transformation and Apoptosis
Cancer Res., November 15, 2004; 64(22): 8208 - 8212.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
D. G. Romero, M. Plonczynski, G. R. Vergara, E. P. Gomez-Sanchez, and C. E. Gomez-Sanchez
Angiotensin II early regulated genes in H295R human adrenocortical cells
Physiol Genomics, September 16, 2004; 19(1): 106 - 116.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
K.-H. Song, Y.-Y. Park, K. C. Park, C. Y. Hong, J. H. Park, M. Shong, K. Lee, and H.-S. Choi
The Atypical Orphan Nuclear Receptor DAX-1 Interacts with Orphan Nuclear Receptor Nur77 and Represses Its Transactivation
Mol. Endocrinol., August 1, 2004; 18(8): 1929 - 1940.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. Val, C. Aigueperse, B. Ragazzon, G. Veyssiere, A.-M. Lefrancois-Martinez, and A. Martinez
Adrenocorticotropin/3',5'-Cyclic AMP-Mediated Transcription of the Scavenger akr1-b7 Gene in Adrenocortical Cells Is Dependent on Three Functionally Distinct Steroidogenic Factor-1-Responsive Elements
Endocrinology, February 1, 2004; 145(2): 508 - 518.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. D. S. A. Wansa, J. M. Harris, G. Yan, P. Ordentlich, and G. E. O. Muscat
The AF-1 Domain of the Orphan Nuclear Receptor NOR-1 Mediates Trans-activation, Coactivator Recruitment, and Activation by the Purine Anti-metabolite 6-Mercaptopurine
J. Biol. Chem., June 27, 2003; 278(27): 24776 - 24790.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Maira, C. Martens, E. Batsche, Y. Gauthier, and J. Drouin
Dimer-Specific Potentiation of NGFI-B (Nur77) Transcriptional Activity by the Protein Kinase A Pathway and AF-1-Dependent Coactivator Recruitment
Mol. Cell. Biol., February 1, 2003; 23(3): 763 - 776.
[Abstract] [Full Text]

Home page
 Circ. Res.Home page
J. Martinez-Gonzalez, J. Rius, A. Castello, C. Cases-Langhoff, and L. Badimon
Neuron-Derived Orphan Receptor-1 (NOR-1) Modulates Vascular Smooth Muscle Cell Proliferation
Circ. Res., January 10, 2003; 92(1): 96 - 103.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. D. S. A. Wansa, J. M. Harris, and G. E. O. Muscat
The Activation Function-1 Domain of Nur77/NR4A1 Mediates Trans-activation, Cell Specificity, and Coactivator Recruitment
J. Biol. Chem., August 30, 2002; 277(36): 33001 - 33011.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K.-H. Song, K. Lee, and H.-S. Choi
Endocrine Disrupter Bisphenol A Induces Orphan Nuclear Receptor Nur77 Gene Expression and Steroidogenesis in Mouse Testicular Leydig Cells
Endocrinology, June 1, 2002; 143(6): 2208 - 2215.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. N. McEvoy, E. A. Murphy, T. Ponnio, O. M. Conneely, B. Bresnihan, O. FitzGerald, and E. P. Murphy
Activation of Nuclear Orphan Receptor NURR1 Transcription by NF-{kappa}B and Cyclic Adenosine 5'-Monophosphate Response Element-Binding Protein in Rheumatoid Arthritis Synovial Tissue
J. Immunol., March 15, 2002; 168(6): 2979 - 2987.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K.-H. Song, J.-I. Park, M.-O. Lee, J. Soh, K. Lee, and H.-S. Choi
LH Induces Orphan Nuclear Receptor Nur77 Gene Expression in Testicular Leydig Cells
Endocrinology, December 1, 2001; 142(12): 5116 - 5123.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J.-I. Park, H.-J. Park, H.-S. Choi, K. Lee, W.-K. Lee, and S.-Y. Chun
Gonadotropin Regulation of NGFI-B Messenger Ribonucleic Acid Expression during Ovarian Follicle Development in the Rat
Endocrinology, July 1, 2001; 142(7): 3051 - 3059.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
N. Hiroi, G. P. Chrousos, B. Kohn, A. Lafferty, M. Abu-Asab, S. Bonat, A. White, and S. R. Bornstein
Adrenocortical-Pituitary Hybrid Tumor Causing Cushing's Syndrome
J. Clin. Endocrinol. Metab., June 1, 2001; 86(6): 2631 - 2637.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2000 by The Endocrine Society