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The gp130 Cytokines Interleukin-11 and Ciliary Neurotropic Factor Regulate through Specific Receptors the Function and Growth of Lactosomatotropic and Folliculostellate Pituitary Cell Lines¹

Laboratorio de Fisiología y Biología Molecular, Department de Biología, FCEN, Universidad de Buenos Aires (C.P.C., A.C.N., E.A.), 1428 Buenos Aires, Argentina; Department of Endocrinology, Max Planck Institute of Psychiatry (M.P.P., P.L., U.R., G.K.S.), 80804 Munich, Germany; Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires (V.G), 1427 Buenos Aires, Argentina; Hospital Santa Lucía (A.C.), 1232 Buenos Aires, Argentina

Address all correspondence and requests for reprints to: Dr. E. Arzt, Laboratorio de Fisiología y Biología Molecular, Department de Biología, Facultad Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, 1428 Buenos Aires, Argentina. E-mail: earzt{at}bg.fcen.uba.ar

Two of the most potent cytokines regulating anterior pituitary cell function are leukemia inhibitory factor and interleukin-6 (IL-6), which belong to the cytokine receptor family using the common gp130 signal transducer. We studied the actions of two other members of this family, IL-11 and ciliary neurotropic factor (CNTF), on folliculostellate (FS) cells (TtT/GF cell line) and lactosomatotropic cells (GH3 cell line). The messenger RNA (mRNA) for the -chain specific for the IL-11 receptor (1.7 kb) and CNTF receptor (2 kb) are expressed on both cell types. In addition, we detected CNTF receptor mRNA in normal rat anterior pituitary cells. IL-11 (1.25–5 nM) dose dependently stimulated the proliferation of FS cells. CNTF, at doses from 0.4–2 nM, also significantly stimulated the growth of these cells. In addition, both cytokines significantly stimulated proliferation of lactosomatotropic GH3 cells, and CNTF stimulated hormone production (GH and PRL) at 24 h by these cells. At 16–72 h, IL-11 stimulates the secretion of the angiogenic factor vascular endothelial growth factor by FS cells. In addition, both GH3 and FS cells express CNTF mRNA. These data suggest that IL-11 and CNTF may act as growth and regulatory factors in anterior pituitary cells.

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