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Leland J. and Dorothy L. Olson Center for Women’s Health, Departments of Obstetrics and Gynecology (S.K.R., L.A.), and Physiology and Biophysics (S.K.R.), University of Nebraska Medical Center, Omaha, Nebraska 68198-4515
Address all correspondence and requests for reprints to: Shyamal K. Roy, BH 3040, Departments of Ob/Gyn and Physiology and Biophysics, University of Nebraska Medical Center, 984515 Nebraska Medical Center, Omaha, Nebraska 68198-4515. E-mail: skroy{at}unmc.edu
Whereas FSH action is critical for the growth of preantral follicles,
its role in the development of primordial follicles is controversial.
The objective of the present study was to evaluate whether perinatal
(fetal through early postnatal) FSH action is needed for the formation
of primordial follicles, which first appear in the hamster ovary on the
7th to 8th day of postnatal life. A single dose of FSH-specific
polyclonal antibody was injected into pregnant hamsters on the 12th,
13th, or 14th day of gestation and into newborn hamsters. Some of the
antibody-exposed postnatal hamsters were injected with a single dose of
equine CG (eCG) to check the reversibility of the antibody action.
Ovaries were collected on D8pn or D12pn, and the percentage of
primordial, primary, and secondary follicles was quantitated
morphometrically. Ovaries of 8-day-old hamsters that were born to
mothers treated with a single sc dose of the anti-FSH-antibody on day
12 of gestation had significantly reduced numbers of primordial
follicles, compared with those treated with preimmune serum or saline
(2.4% vs. 25%); however, the antibody inhibition was
nearly completely reversed (
18%) by a single injection of eCG on
the first day of life. Delaying antibody treatment during late
gestation caused a time-dependent block in granulosa cell
differentiation, with a consequent proportional increase in the
percentage of primordial follicles. This indicates that FSH-induction
of primordial follicle development begins at a critical time of ovarian
development. On the other hand, shortening the postnatal duration of
eCG exposure reduced the degree of reversal, suggesting that prolonged
perinatal FSH action is essential for developing the full gamut of
primordial follicles. These results provide the first direct evidence
that FSH action during fetal ovarian development is critical for the
onset of primordial follicle formation.
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P. Yang, J. Wang, Y. Shen, and S. K. Roy Developmental Expression of Estrogen Receptor (ER) {alpha} and ER{beta} in the Hamster Ovary: Regulation by Follicle-Stimulating Hormone Endocrinology, December 1, 2004; 145(12): 5757 - 5766. [Abstract] [Full Text] [PDF]
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J. Wang and S. K. Roy Growth Differentiation Factor-9 and Stem Cell Factor Promote Primordial Follicle Formation in the Hamster: Modulation by Follicle-Stimulating Hormone Biol Reprod, March 1, 2004; 70(3): 577 - 585. [Abstract] [Full Text] [PDF]
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