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Transforming Growth Factor-ß1-Induced Proliferation of the Prostate Cancer Cell Line, TSU-Pr1: The Role of Platelet-Derived Growth Factor¹

Department of Urology, Northwestern University Medical School, Chicago, Illinois 60611

Address all correspondence and requests for reprints to: Dr. Chung Lee, Department of Urology, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, Illinois 60611.

The results of our previous study revealed that transforming growth factor-ß1 (TGFß1) stimulated proliferation of the prostate cancer cell line, TSU-Pr1. This observation is unexpected, for TGFß usually inhibits proliferation in prostate cancer cells. The present study examines possible mechanisms through which TGFß1 induces this proliferation. We postulate that TGFß1 action is mediated through an indirect mechanism by inducing the expression of platelet-derived growth factor (PDGF), which, in turn, stimulates proliferation. The TGFß1-induced proliferation can be abrogated by treatment with a PDGF-neutralizing antibody. Treatment with exogenous PDGF significantly increased TSU-Pr1 proliferation. Finally, treatment of TSU-Pr1 cells with TGFß1 resulted in an increase in PDGF secretion. These results indicate that TGFß1-induced proliferation in TSU-Pr1 cells is at least mediated through an increased secretion of PDGF.

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