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Growth Hormone (GH) and Prolactin Receptors in Human Peripheral Blood Mononuclear Cells: Relation with Age and GH-Binding Protein¹

INSERM Unité 344, Molecular Endocrinology (S.J., C.K., M.-C.P.-V.), Centre d’Investigation Clinique, Hôpital Necker (J.-L.B., N.B.), and Université Paris V, CNRS URA 1461 (M.-C.G., M.D.), Hôpital Necker, Paris Cedex 15, 75730 France; Laboratory of Molecular Biology and Genetic Engineering, Université de Liège, Belgium (S.K.); Department of Medicine (Z.W.), Ludwig Maximilians Universität, Munich 80336, Germany

Address all correspondence and requests for reprints to: Dr. M.-C. Postel-Vinay, INSERM Unité 344, Faculté de Médecine Necker, 156 rue de Vaugirard, 75730 Paris Cedex 15, France. E-mail: postel-vinay{at}necker.fr

GH receptors (GHRs) and PRL receptors (PRLRs) were studied in human peripheral blood mononuclear cells (PBMC) using flow cytometry, biotinylated anti-GH receptor monoclonal antibody 10B8, and biotinylated human PRL. Variations of GHR and PRLR expression and the relationship of plasma GHBP and GH receptor in PBMC subsets were examined as a function of age and sex. By double immunofluorescence staining, we show that about 30% of total cells express GH receptors, with a low expression in T cells, whereas almost all B cells and monocytes are GH receptor positive. Four age groups were defined among the 64 normal volunteers, aged 12 to 85 yr, who were included in the study. The percentage of PBMC expressing GH receptors is significantly lower in group 2 (20–40 yr) than in group 1 (12–20 yr) and group 4 (>60 yr). In T cells, monocytes and B cells, no significant changes are detected in either the percentage of GH receptor positive cells or in the GH receptor level per cell. The level of PRLRs expressed in PBMC is significantly higher in age group 2 than in age group 4. A negative correlation is observed between plasma GHBP and the percentage of PBMC expressing GH receptors. These results suggest that regulation of GH receptors in lymphocytes and in other target cells could be different.

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