This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Misiti, S. Articles by Chin, W. W. Search for Related Content PubMed PubMed Citation Articles by Misiti, S. Articles by Chin, W. W.

Expression of Steroid Receptor Coactivator-1 mRNA in the Developing Mouse Embryo: A Possible Role in Olfactory Epithelium Development

Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School (S.M., N.K., M.B., W.W.C.), Boston, Massachusetts 02115; and Molecular Oncogenesis Laboratory (S.M., A.F.), Regina Elena Cancer Institute; Rome, Italy

Address all correspondence and requests for reprints to William W. Chin, M.D., Brigham & Women’s Hospital, G. W. Thorn Research Building, Room 1019, 20 Shattuck Street, Department of Medicine, Boston, Massachusetts 02115.

Ligand-dependent nuclear hormone receptors (NRs), such as retinoic acid and thyroid hormone receptors, play critical roles in diverse aspects of development. They enhance or repress transcription by recruiting an array of coactivator and corepressor proteins, which function as signaling intermediates between the NRs and the basal transcriptional machinery. To study the possible involvement of these cofactors on tissue-specific regulation of gene expression by NRs, we examined the expression of the coactivator SRC-1 mRNA during mouse enbryogenesis by in situ hydridization (ISH). ³⁵S-labeled riboprobe specific for SRC-1 mRNA was used for analysis. The distribution of this transcript was studied from 8.5 to 18.5 embryonic days (E8.5–E18.5) and in postnatal day 15 (P15). The SRC-1 transcript was largely ubiquitously expressed, even on E8.5. At E14.5 and E18.5, highest levels of SRC-1 transcript was found in the olfactory epithelium. Significant SRC-1 hybridization signal was also detected in the neocortex, anterior pituitary and heart. We conclude that (1) SRC-1 mRNA is widely expressed in the developing embryo, and (2) SRC-1 mRNA is expressed at the highest level in the olfactory epithelium, suggesting that this coactivator may be involved in the development and/or function of the olfactory system.

This article has been cited by other articles:

				P. Cano, A. Godoy, R. Escamilla, R. Dhir, and S. A. Onate Stromal-Epithelial Cell Interactions and Androgen Receptor-Coregulator Recruitment Is Altered in the Tissue Microenvironment of Prostate Cancer Cancer Res., January 15, 2007; 67(2): 511 - 519. [Abstract] [Full Text] [PDF]

				J.-W. Jeong, I. Kwak, K. Y. Lee, L. D. White, X.-P. Wang, F. C. Brunicardi, B. W. O'Malley, and F. J. DeMayo The Genomic Analysis of the Impact of Steroid Receptor Coactivators Ablation on Hepatic Metabolism Mol. Endocrinol., May 1, 2006; 20(5): 1138 - 1152. [Abstract] [Full Text] [PDF]

				J. N. Winnay, J. Xu, B. W. O'Malley, and G. D. Hammer Steroid Receptor Coactivator-1-Deficient Mice Exhibit Altered Hypothalamic-Pituitary-Adrenal Axis Function Endocrinology, March 1, 2006; 147(3): 1322 - 1332. [Abstract] [Full Text] [PDF]

				J. Igarashi-Migitaka, A. Takeshita, N. Koibuchi, S. Yamada, R. Ohtani-Kaneko, and K. Hirata Differential expression of p160 steroid receptor coactivators in the rat testis and epididymis Eur. J. Endocrinol., October 1, 2005; 153(4): 595 - 604. [Abstract] [Full Text] [PDF]

				E. Setiawan, D. Owen, L. McCabe, A. Kostaki, M. H. Andrews, and S. G. Matthews Glucocorticoids Do Not Alter Developmental Expression of Hippocampal or Pituitary Steroid Receptor Coactivator-1 and -2 in the Late Gestation Fetal Guinea Pig Endocrinology, August 1, 2004; 145(8): 3796 - 3803. [Abstract] [Full Text] [PDF]

				M. Mark, H. Yoshida-Komiya, M. Gehin, L. Liao, M.-J. Tsai, B. W. O'Malley, P. Chambon, and J. Xu Partially redundant functions of SRC-1 and TIF2 in postnatal survival and male reproduction PNAS, March 30, 2004; 101(13): 4453 - 4458. [Abstract] [Full Text] [PDF]

				H. A. Molenda, C. P. Kilts, R. L. Allen, and M. J. Tetel Nuclear Receptor Coactivator Function in Reproductive Physiology and Behavior Biol Reprod, November 1, 2003; 69(5): 1449 - 1457. [Abstract] [Full Text] [PDF]

				J. Xu and Q. Li Review of the in Vivo Functions of the p160 Steroid Receptor Coactivator Family Mol. Endocrinol., September 1, 2003; 17(9): 1681 - 1692. [Abstract] [Full Text] [PDF]

				E. Nishihara, H. Yoshida-Komiya, C.-S. Chan, L. Liao, R. L. Davis, B. W. O'Malley, and J. Xu SRC-1 Null Mice Exhibit Moderate Motor Dysfunction and Delayed Development of Cerebellar Purkinje Cells J. Neurosci., January 1, 2003; 23(1): 213 - 222. [Abstract] [Full Text] [PDF]

				H. A. Molenda, A. L. Griffin, A. P. Auger, M. M. McCarthy, and M. J. Tetel Nuclear Receptor Coactivators Modulate Hormone-Dependent Gene Expression in Brain and Female Reproductive Behavior in Rats Endocrinology, February 1, 2002; 143(2): 436 - 444. [Abstract] [Full Text] [PDF]

				O. C. Meijer, P. J. Steenbergen, and E. R. de Kloet Differential Expression and Regional Distribution of Steroid Receptor Coactivators SRC-1 and SRC-2 in Brain and Pituitary Endocrinology, June 1, 2000; 141(6): 2192 - 2199. [Abstract] [Full Text] [PDF]

				J. Xu, L. Liao, G. Ning, H. Yoshida-Komiya, C. Deng, and B. W. O'Malley The steroid receptor coactivator SRC-3 (p/CIP/RAC3/AIB1/ACTR/TRAM-1) is required for normal growth, puberty, female reproductive function, and mammary gland development PNAS, June 6, 2000; 97(12): 6379 - 6384. [Abstract] [Full Text] [PDF]