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Evidence for Stanniocalcin Gene Expression in Mammalian Bone¹

Department of Oral Anatomy (Y.Y., A.S., S.M., N.M.), Hiroshima University School of Dentistry, Minami-ku, Hiroshima 734-8553, Japan; and R & D Center, BML, Inc. (A.I., S.T., J.H.), Kawagoe, Saitama 350-1101, Japan

Address all correspondence and requests for reprints to: Norihiko Maeda, Department of Anatomy, Hiroshima University School of Dentistry, Minami-ku, Hiroshima 734-8553, Japan.

Stanniocalcin (STC) acts as a regulator of calcium and phosphate homeostasis in an endocrine manner in bony fish. Recently, complementary DNAs encoding human and mouse STC have been characterized, and the messenger RNA (mRNA) expression was identified in various tissues, such as kidney, small intestine, prostate, thyroid, and ovary. Because previous studies concerning the effects of fish STC on mammalian bone have been discussed, there is a good possibility that mammalian STC is a local factor in bone. Here, we demonstrated STC mRNA expression in neonatal mouse calvaria, the primary cultured mouse osteoblast-rich fractions, and human and mouse osteoblastic cell lines. We also mapped the cellular distribution of the STC mRNA in femur and calvaria in developing mice. Several transcripts with a major 4-kb band were detected in all samples. The cellular distribution of the mRNA expression corresponded closely to osteoblasts in both femur and calvaria. Significant labeling of the STC mRNA was also identified in chondrocytes but not in osteoclasts and other bone marrow elements. These results are the first evidence that hormone may be actually expressed in osteoblasts and chondrocytes, and they strongly implicate the involvement of local STC in both endochondral and membrane bone as an autocrine/paracrine factor.

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