This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Adachi, K. Articles by Oka, T. Search for Related Content PubMed PubMed Citation Articles by Adachi, K. Articles by Oka, T.

Hormonal Induction of Mouse Selenocysteine Transfer Ribonucleic Acid (tRNA) Gene Transcription-Activating Factor and Its Functional Importance in the Selenocysteine tRNA Gene Transcription in Mouse Mammary Gland

Laboratory of Molecular and Cellular Biology, National Institutes of Diabetes, Digestive, and Kidney Disease, National Institutes of Health, Bethesda, Maryland 20892

Address all correspondence and requests for reprints to: LMCB/NIDDK/NIH, Building 8, Room 309, Bethesda, Maryland 20892.

Mouse selenocysteine transfer RNA (tRNA) gene transcription-activating factor (mStaf) is a transcriptional activator that enhances RNA polymerase III-dependent mouse selenocysteine tRNA (tRNA^Sec) gene transcription. The DNA-binding activity of mStaf in mouse mammary gland undergoes developmental changes, reaching a maximal level during the period of lactation. In this study, we employed an organ culture system to examine the hormonal regulation of mStaf binding and its role in the tRNA^Sec transcription in the mammary gland. The results showed that mStaf binding in mammary explants was stimulated by treatment with the lactogenic hormones, PRL, insulin, and hydrocortisone and that a specific MEK inhibitor, PD98059, inhibited the hormonal stimulation of mStaf binding. Other kinase inhibitors, such as a Janus kinase inhibitor and a calmodulin kinase inhibitor, had no apparent effect. Northern and Western blot analyses revealed that the level of both mStaf messenger RNA and protein was enhanced by the lactogenic hormones and was reduced by the concomitant treatment with PD98059. The mitogen-activated protein kinase activity in cultured explants was rapidly induced and maintained at high levels by the lactogenic hormones. We also found that the lactogenic hormones increased the amount of tRNA^Sec in a time-dependent manner, which followed the increase in mStaf binding in cultured mammary explants. These results support the view that mStaf plays a key role in the hormonal stimulation of tRNA^Sec transcription in the mammary gland.

This article has been cited by other articles:

				K. Bruzelius, T. Hoac, R. Sundler, G. Onning, and B. Akesson Occurrence of Selenoprotein Enzyme Activities and mRNA in Bovine Mammary Tissue J Dairy Sci, February 1, 2007; 90(2): 918 - 927. [Abstract] [Full Text] [PDF]

				J. Kohrle, F. Jakob, B. Contempre, and J. E. Dumont Selenium, the Thyroid, and the Endocrine System Endocr. Rev., December 1, 2005; 26(7): 944 - 984. [Abstract] [Full Text] [PDF]

				C. E. Grossman, Y. Qian, K. Banki, and A. Perl ZNF143 Mediates Basal and Tissue-specific Expression of Human Transaldolase J. Biol. Chem., March 26, 2004; 279(13): 12190 - 12205. [Abstract] [Full Text] [PDF]

				Y. Yamazaki, H. Kubota, M. Nozaki, and K. Nagata Transcriptional Regulation of the Cytosolic Chaperonin {theta} Subunit Gene, Cctq, by Ets Domain Transcription Factors Elk-1, Sap-1a, and Net in the Absence of Serum Response Factor J. Biol. Chem., August 15, 2003; 278(33): 30642 - 30651. [Abstract] [Full Text] [PDF]