help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shenoy, U. V.
Right arrow Articles by Sumners, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shenoy, U. V.
Right arrow Articles by Sumners, C.
Endocrinology Vol. 140, No. 1 500-509
Copyright © 1999 by The Endocrine Society

ARTICLES

Angiotensin II Type 2 Receptor-Mediated Apoptosis of Cultured Neurons from Newborn Rat Brain1

Ujjwala V. Shenoy, Elaine M. Richards, Xian-Cheng Huang and Colin Sumners

Department of Physiology, College of Medicine and the University of Florida Brain Institute, University of Florida, Gainesville, Florida 32610

Address all correspondence and requests for reprints to: Dr. Colin Sumners, Department of Physiology, P.O. Box 100274, 1600 S.W. Archer Road, University of Florida, Gainesville, Florida 32610. E-mail: csumners{at}phys.med.ufl.edu

Angiotensin II (Ang II) type 2 (AT2) receptors are highly expressed in neonate brain and may have a role in developmental processes such as apoptosis. Concurrent activation of c-Jun N-terminal kinase (JNK) and inhibition of Erk mitogen-activated protein kinase activities is important for apoptosis in many cells, and we previously demonstrated that stimulation of AT2 receptors causes decreased mitogen-activated protein kinase activity in neurons cultured from newborn rat hypothalamus and brain stem. Using such cultures we have employed terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick end labeling and internucleosomal DNA fragmentation to assess the role of AT2 receptors in neuronal apoptosis. Ang II (100 nM; 4–72 h) alone produced no significant neuronal apoptosis, and AT2 receptor activation did not stimulate JNK activity. However, exposure of cultures to UV radiation (6 J/m2/sec for 4 sec) to stimulate JNK elicited neuronal apoptosis that was significantly enhanced by Ang II, an effect that was abolished by the AT2 receptor antagonist PD 123,319 (1 µM) or the serine/threonine phosphatase inhibitor okadaic acid (3 nM). Additionally, Ang II enhanced the UV radiation-induced decrease in the levels of the DNA repair enzyme poly-(ADP-ribose) polymerase. These data indicate that Ang II, via AT2 receptors and activation of a serine/threonine phosphatase, contributes to neuronal apoptosis.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
E. Q. Haxhija, H. Yang, A. U. Spencer, H. Koga, X. Sun, and D. H. Teitelbaum
Modulation of mouse intestinal epithelial cell turnover in the absence of angiotensin converting enzyme
Am J Physiol Gastrointest Liver Physiol, July 1, 2008; 295(1): G88 - G98.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. Paul, A. Poyan Mehr, and R. Kreutz
Physiology of local Renin-Angiotensin systems.
Physiol Rev, July 1, 2006; 86(3): 747 - 803.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
T. Matsuura, H. Kumagai, H. Onimaru, A. Kawai, K. Iigaya, T. Onami, K. Sakata, N. Oshima, T. Sugaya, and T. Saruta
Electrophysiological Properties of Rostral Ventrolateral Medulla Neurons in Angiotensin II 1a Receptor Knockout Mice
Hypertension, August 1, 2005; 46(2): 349 - 354.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
C. J. Wruck, H. Funke-Kaiser, T. Pufe, H. Kusserow, M. Menk, J. H. Schefe, M. L. Kruse, M. Stoll, and T. Unger
Regulation of Transport of the Angiotensin AT2 Receptor by a Novel Membrane-Associated Golgi Protein
Arterioscler. Thromb. Vasc. Biol., January 1, 2005; 25(1): 57 - 64.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
L. Wu, M. Iwai, Z. Li, T. Shiuchi, L.-J. Min, T.-X. Cui, J.-M. Li, M. Okumura, C. Nahmias, and M. Horiuchi
Regulation of Inhibitory Protein-{kappa}B and Monocyte Chemoattractant Protein-1 by Angiotensin II Type 2 Receptor-Activated Src Homology Protein Tyrosine Phosphatase-1 in Fetal Vascular Smooth Muscle Cells
Mol. Endocrinol., March 1, 2004; 18(3): 666 - 678.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-y. Kaimori, M. Takenaka, H. Nakajima, T. Hamano, M. Horio, T. Sugaya, T. Ito, M. Hori, K. Okubo, and E. Imai
Induction of Glia Maturation Factor-{beta} in Proximal Tubular Cells Leads to Vulnerability to Oxidative Injury through the p38 Pathway and Changes in Antioxidant Enzyme Activities
J. Biol. Chem., August 29, 2003; 278(35): 33519 - 33527.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
H. Yang, G. Shaw, and M. K. Raizada
ANG II stimulation of neuritogenesis involves protein kinase B in brain neurons
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2002; 283(1): R107 - R114.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. Stoll, A. W.A. Hahn, U. Jonas, Y. Zhao, B. Schieffer, J. W. Fischer, and T. Unger
Identification of a Zinc Finger Homoeodomain Enhancer Protein After AT2 Receptor Stimulation by Differential mRNA Display
Arterioscler. Thromb. Vasc. Biol., February 1, 2002; 22(2): 231 - 237.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
L. Wise-Faberowski, M. K. Raizada, and C. Sumners
Oxygen and Glucose Deprivation-Induced Neuronal Apoptosis is Attenuated by Halothane and Isoflurane
Anesth. Analg., November 1, 2001; 93(5): 1281 - 1287.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. Yang, X. Wang, and M. K. Raizada
Characterization of Signal Transduction Pathway in Neurotropic Action of Angiotensin II in Brain Neurons
Endocrinology, August 1, 2001; 142(8): 3502 - 3511.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
H. Sugino, R. Ozono, S. Kurisu, H. Matsuura, M. Ishida, T. Oshima, M. Kambe, Y. Teranishi, H. Masaki, and H. Matsubara
Apoptosis Is Not Increased in Myocardium Overexpressing Type 2 Angiotensin II Receptor in Transgenic Mice
Hypertension, June 1, 2001; 37(6): 1394 - 1398.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. Zhu, C. Sumners, C. H. Gelband, and P. Posner
Chronotropic Effect of Angiotensin II via Type 2 Receptors in Rat Brain Neurons
J Neurophysiol, May 1, 2001; 85(5): 2177 - 2183.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
T.-X. Cui, H. Nakagami, M. Iwai, Y. Takeda, T. Shiuchi, L. Daviet, C. Nahmias, and M. Horiuchi
Pivotal role of tyrosine phosphatase SHP-1 in AT2 receptor-mediated apoptosis in rat fetal vascular smooth muscle cell
Cardiovasc Res, March 1, 2001; 49(4): 863 - 871.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Breault, E. Chamoux, J.-G. LeHoux, and N. Gallo-Payet
Localization of G Protein {{alpha}}-Subunits in the Human Fetal Adrenal Gland
Endocrinology, December 1, 2000; 141(12): 4334 - 4341.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. Zhu, R. Natarajan, J. L. Nadler, J. M. Moore, C. H. Gelband, and C. Sumners
Angiotensin II Increases Neuronal Delayed Rectifier K+ Current: Role of 12-Lipoxygenase Metabolites of Arachidonic Acid
J Neurophysiol, November 1, 2000; 84(5): 2494 - 2501.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. W. CARLEY, K. BERECEK, A. VIDENOVIC, and M. RADULOVACKI
Sleep-disordered Respiration in Phenotypically Normotensive, Genetically Hypertensive Rats
Am. J. Respir. Crit. Care Med., October 1, 2000; 162(4): 1474 - 1479.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. Busche, S. Gallinat, R.-M. Bohle, A. Reinecke, J. Seebeck, F. Franke, L. Fink, M. Zhu, C. Sumners, and T. Unger
Expression of Angiotensin AT1 and AT2 Receptors in Adult Rat Cardiomyocytes after Myocardial Infarction : A Single-Cell Reverse Transcriptase-Polymerase Chain Reaction Study
Am. J. Pathol., August 1, 2000; 157(2): 605 - 611.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
N. Elbaz, K. Bedecs, M. Masson, M. Sutren, A. D. Strosberg, and C. Nahmias
Functional Trans-inactivation of Insulin Receptor Kinase by Growth-Inhibitory Angiotensin II AT2 Receptor
Mol. Endocrinol., June 1, 2000; 14(6): 795 - 804.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. Gallinat, S. Busche, M. K. Raizada, and C. Sumners
The angiotensin II type 2 receptor: an enigma with multiple variations
Am J Physiol Endocrinol Metab, March 1, 2000; 278(3): E357 - E374.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
E. Chamoux
Involvement of the Angiotensin II Type 2 Receptor in Apoptosis during Human Fetal Adrenal Gland Development
J. Clin. Endocrinol. Metab., December 1, 1999; 84(12): 4722 - 4730.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
F. Cote, T. H. Do, L. Laflamme, J.-M. Gallo, and N. Gallo-Payet
Activation of the AT2 Receptor of Angiotensin II Induces Neurite Outgrowth and Cell Migration in Microexplant Cultures of the Cerebellum
J. Biol. Chem., October 29, 1999; 274(44): 31686 - 31692.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1999 by The Endocrine Society