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Endocrinology, Vol 137, 1049-1056, Copyright © 1996 by Endocrine Society

ARTICLES

Osteopetrotic (op/op) mice are unable to maintain serum calcium levels despite hyperabsorption of calcium

LC McCary and HF DeLuca
Department of Biochemistry, University of Wisconsin-Madison, Wisconsin 53706, USA.

The effect of vitamin D on serum calcium, vitamin D receptor (VDR) protein level, and fate of radioactive calcium ingested were compared in vitamin D-deficient osteopetrotic (op/op) mice and their wild-type vitamin D-deficient littermates. Vitamin D deficiency was achieved in mice after feeding them a vitamin D-deficient diet for 5-6 weeks. Serum calcium did not increase in op/op mice in response to 1,25- dihydroxyvitamin D3. Furthermore, op/op mice were not capable of increasing serum calcium levels by passively absorbing calcium from the diet. These defects in calcium homeostasis were neither the result of the inability of the VDR to bind its ligand, as determined by the hydroxylapatite assay, nor the result of abnormal regulation of intestinal VDR, as determined by the enzyme-linked immunosorbent assay. Upon administration of radioactive calcium by oral gavage, it was found that vitamin D-deficient, op/op mice had an extremely efficient mechanism to absorb dietary calcium, but the calcium absorbed was readily shunted into bone; thus serum calcium did not increase. In addition, op/op mice also possessed vitamin D-stimulated intestinal calcium absorption, but this process was muted by the overwhelming vitamin D-independent mechanism of dietary calcium absorption.


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 Am. J. Physiol. Endocrinol. Metab.Home page
K. B. Williams and H. F. DeLuca
Characterization of intestinal phosphate absorption using a novel in vivo method
Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1917 - E1921.
[Abstract] [Full Text] [PDF]


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