Endocrinology, Vol 136, 2040-2046, Copyright © 1995 by Endocrine Society

ARTICLES

Cyclic adenosine 3',5'-monophosphate stimulation of placental proliferin and proliferin-related protein secretion

M Yamaguchi, T Imai, T Maeda, M Sakata, A Miyake and DI Linzer
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.

To identify factors that regulate proliferin (PLF) and PLF-related protein (PRP) secretion by the mouse placenta, placental cells from day 9 of pregnancy were cultured for up to 5 days, and PLF and PRP release into the medium was assessed by RIA. Transforming growth factor-alpha, interleukin-1 alpha, and interleukin-6 did not regulate either PLF or PRP secretion. However, treatment of primary placental cell cultures with 8-bromo-cAMP, cholera toxin, or forskolin resulted in 2- to 3-fold increases in the percentages of PLF- and PRP-producing cells in the population and corresponding increases in both PLF and PRP messenger RNA and secreted protein. The increase in the number of PLF-producing cells was accompanied by an increase in the number of cells expressing both PLF and mouse placental lactogen-I. These data suggest that cAMP levels can regulate trophoblast giant cell differentiation and, consequently, the amount of PLF and PRP secretion.

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