Endocrinology, Vol 136, 2033-2039, Copyright © 1995 by Endocrine Society

ARTICLES

Kaliuretic peptide: the most potent inhibitor of Na(+)-K+ ATPase of the atrial natriuretic peptides

S Chiou and DL Vesely
Department of Medicine, University of South Florida Health Sciences Center, Tampa, USA.

The present investigation was designed to determine whether atrial natriuretic peptides consisting of amino acids 1-30 (i.e. long-acting natriuretic peptide), 31-67 (vessel dilator), 79-98 (kaliuretic peptide), and 99-126 [atrial natriuretic factor (ANF)] of the 126 amino acid ANF prohormone inhibit sodium-potassium-ATPase as part of their mechanism(s) of action for producing a natriuresis and/or kaliuresis. Kaliuretic peptide, long-acting natriuretic peptide, vessel dilator and ANF at their 10(-11) M concentrations inhibited Na(+)-K(+)-ATPase 39.5%, 27.8%, 19.2%, and 4% respectively, in bovine renal medulla, whereas their inhibition in renal cortical membranes was 37.5%, 27.5%, 20%, and 0%, respectively. Ouabain (0.5 mM) inhibited kidney medullary Na(+)-K(+)-ATPase 45% and in the cortex, 38%. There was no additive effect of any of these peptides with ouabain suggesting that they are interacting with the same site on the Na(+)-K(+)-ATPase as ouabain. To help elucidate the mechanism of these peptides' interaction with Na(+)- K(+)-ATPase, naproxen (0.5 mM), an inhibitor of prostaglandin synthesis, and direct measurement of prostaglandin E2 by RIA were used. Naproxen completely blocked the inhibition of Na(+)-K(+)-ATPase by kaliuretic peptide, long-acting natriuretic peptide, and vessel dilator suggesting that their inhibition of Na(+)-K(+)-ATPase in both the kidney medulla and cortex are mediated by prostaglandins. Direct measurement of prostaglandin E2 revealed that kaliuretic peptide > long- acting natriuretic peptide > vessel dilator increased prostaglandin E2 synthesis, whereas ANF did not have any effect. Of interest, angiotensin II and ouabain inhibition of Na(+)-K(+)-ATPase were also completely blocked by naproxen.

This article has been cited by other articles:

				O. V. Fedorova, N. I. Agalakova, C. H. Morrell, E. G. Lakatta, and A. Y. Bagrov ANP Differentially Modulates Marinobufagenin-Induced Sodium Pump Inhibition in Kidney and Aorta Hypertension, December 1, 2006; 48(6): 1160 - 1168. [Abstract] [Full Text] [PDF]

				H. Patel, J. R. Dietz, A. Owen, G. S. Miguel, M. T. McCormick, D. D. Schocken, and D. L. Vesely Combined Treatment with Vessel Dilator and Kaliuretic Hormone in Persons with Congestive Heart Failure Experimental Biology and Medicine, June 1, 2004; 229(6): 521 - 527. [Abstract] [Full Text] [PDF]

				D. L. Vesely Natriuretic peptides and acute renal failure Am J Physiol Renal Physiol, August 1, 2003; 285(2): F167 - F177. [Abstract] [Full Text] [PDF]

				D. L Vesely Atrial natriuretic peptides in pathophysiological diseases Cardiovasc Res, September 1, 2001; 51(4): 647 - 658. [Full Text] [PDF]

				J. R. Dietz, D. Y. Scott, C. S. Landon, and S. J. Nazian Evidence supporting a physiological role for proANP-(1-30) in the regulation of renal excretion Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2001; 280(5): R1510 - R1517. [Abstract] [Full Text] [PDF]

				L. C. Clark, H. Farghaly, S. R. Saba, and D. L. Vesely Amelioration with vessel dilator of acute tubular necrosis and renal failure established for 2 days Am J Physiol Heart Circ Physiol, May 1, 2000; 278(5): H1555 - H1564. [Abstract] [Full Text] [PDF]

				C. Zellner, A. A. Protter, E. Ko, M. R. Pothireddy, T. DeMarco, S. J. Hutchison, T. M. Chou, K. Chatterjee, and K. Sudhir Coronary vasodilator effects of BNP: mechanisms of action in coronary conductance and resistance arteries Am J Physiol Heart Circ Physiol, March 1, 1999; 276(3): H1049 - H1057. [Abstract] [Full Text] [PDF]

				D. L. Vesely, J. R. Dietz, J. R. Parks, M. Baig, M. T. McCormick, G. Cintron, and D. D. Schocken Vessel Dilator Enhances Sodium and Water Excretion and Has Beneficial Hemodynamic Effects in Persons With Congestive Heart Failure Circulation, July 28, 1998; 98(4): 323 - 329. [Abstract] [Full Text] [PDF]

				B. H. Ackerman, R. M. Overton, M. T. McCormick, D. D. Schocken, and D. L. Vesely Disposition of Vessel Dilator and Long-Acting Natriuretic Peptide in Healthy Humans after a One-Hour Infusion J. Pharmacol. Exp. Ther., August 1, 1997; 282(2): 603 - 608. [Abstract] [Full Text]