help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weinstein, S. P.
Right arrow Articles by Haber, R. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weinstein, S. P.
Right arrow Articles by Haber, R. S.

Endocrinology, Vol 135, 649-654, Copyright © 1994 by Endocrine Society

ARTICLES

Regulation of GLUT2 glucose transporter expression in liver by thyroid hormone: evidence for hormonal regulation of the hepatic glucose transport system

SP Weinstein, E O'Boyle, M Fisher and RS Haber
Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029.

The extent to which the glucose transport system in hepatocytes is regulated in states of altered hepatic glucose metabolism is unclear. Because thyroid hormone is known to increase hepatic glucose output, we hypothesized that thyroid hormone might up-regulate expression of the principal hepatic glucose transporter, GLUT2, facilitating increased glucose efflux across the hepatocyte plasma membrane. GLUT2 protein concentration in crude liver membranes was twice as high in chronically hyperthyroid vs. hypothyroid animals, with intermediate levels in euthyroid controls. Similar results were obtained for total GLUT2 protein, measured in detergent extracts of liver. Northern analysis of total liver RNA demonstrated parallel changes in GLUT2 messenger RNA (mRNA) concentration per g tissue (hypothyroid, 76 +/- 6%; euthyroid, 100 +/- 11%; hyperthyroid, 158 +/- 12%; data expressed as percentage of mean euthyroid values). The daily administration of a large dose of T3 (100 micrograms/100 g BW) to hypothyroid rats caused a prompt increase in hepatic GLUT2 mRNA concentration (2.5-fold at 1 day), but only a modest and gradual change in hepatic GLUT2 protein concentration (+40% at 4 days), suggesting that the GLUT2 protein in liver may have a long half-life. We conclude that thyroid hormone regulates hepatic GLUT2 mRNA and protein expression. Up-regulation of GLUT2 protein expression by thyroid hormone may serve to facilitate increased hepatic glucose output. These results suggest that the hepatic GLUT2 glucose transporter, like the enzymes of gluconeogenesis and glycolysis, is indeed a regulatory target for hormones that control hepatic glucose metabolism.


This article has been cited by other articles:


Home page
 Stem CellsHome page
I. Drobinskaya, T. Linn, T. Saric, R. G. Bretzel, H. Bohlen, J. Hescheler, and E. Kolossov
Scalable Selection of Hepatocyte- and Hepatocyte Precursor-Like Cells from Culture of Differentiating Transgenically Modified Murine Embryonic Stem Cells
Stem Cells, September 1, 2008; 26(9): 2245 - 2256.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y.-Y. Liu, R. S. Heymann, F. Moatamed, J. J. Schultz, D. Sobel, and G. A. Brent
A Mutant Thyroid Hormone Receptor {alpha} Antagonizes Peroxisome Proliferator-Activated Receptor {alpha} Signaling in Vivo and Impairs Fatty Acid Oxidation
Endocrinology, March 1, 2007; 148(3): 1206 - 1217.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1994 by The Endocrine Society