help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Haavisto, A. M.
Right arrow Articles by Huhtaniemi, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haavisto, A. M.
Right arrow Articles by Huhtaniemi, I.

Endocrinology, Vol 132, 1687-1691, Copyright © 1993 by Endocrine Society

ARTICLES

A supersensitive immunofluorometric assay for rat luteinizing hormone

AM Haavisto, K Pettersson, M Bergendahl, A Perheentupa, JF Roser and I Huhtaniemi
Department of Physiology, University of Turku, Finland.

We have developed an immunofluorometric assay (IFMA) for rat (r) LH, which is based on two monoclonal antibodies, one to bovine and the other to human LH. Signal detection occurs by time-resolved fluorescence evoked by a europium label (Delfia, Wallac). The method is fast in comparison to the standard RIA with the NIDDK reagents (4 h vs. 3 days). The sensitivity of the IFMA assay (0.75 pg/tube; NIDDK rLH RP- 2) is over 30-fold higher than that of the NIDDK RIA (usual detection limit, 20-30 pg/tube). Using 25-microliters serum samples, the sensitivity of IFMA is 0.03 micrograms/liter; with 100-microliters samples, it is 0.0075 micrograms/liter. The cross-reactivity of the IFMA assay is 0.3% with rFSH, 3% with rTSH, and less than 0.05% with rGH, rPRL, and the rat alpha-subunit. A linear correlation between IFMA and RIA values is seen at serum levels above 0.4 micrograms/liter. Below this level, only IFMA is able to detect concentration differences between samples. In practice, this means that only IFMA is able to provide meaningful measurements of suppressed levels of serum LH. The correlation coefficient between IFMA and the mouse interstitial cell in vitro bioassay for LH in randomly selected rat pituitary homogenates was 0.93 (n = 47). The serum concentration of LH determined by IFMA is 0.57 +/- 0.10 micrograms/liter in intact adult male rats (mean +/- SEM; n = 12) and 0.41 +/- 0.10 micrograms/liter (n = 10) in randomly cycling females. The level in hypophysectomized rat serum is 0.035 +/- 0.0033 micrograms/liter (n = 8), if the limit of sensitivity (0.03 microgram/liter) is assigned to unmeasurable levels. One-week treatment of male rats with 2-cm Silastic implants containing testosterone suppressed serum LH, measured by IFMA, from 0.56 +/- 0.057 to 0.086 +/- 0.057 micrograms/liter (P < 0.01). The suppression of LH measured in the same samples by RIA was lower, from 0.73 +/- 0.057 to 0.44 +/- 0.048 micrograms/liter (P < 0.01). A 5-day starvation of intact male rats suppressed serum LH from 0.57 +/- 0.10 to 0.30 +/- 0.05 microgram/liter by IFMA (P < 0.01), whereas the decrease determined by RIA was not significant (0.80 +/- 0.07 vs. 0.66 +/- 0.13 micrograms/liter).(ABSTRACT TRUNCATED AT 400 WORDS)


This article has been cited by other articles:


Home page
 EndocrinologyHome page
C. A. Christian and S. M. Moenter
Critical Roles for Fast Synaptic Transmission in Mediating Estradiol Negative and Positive Feedback in the Neural Control of Ovulation
Endocrinology, November 1, 2008; 149(11): 5500 - 5508.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. A. Christian, C. Glidewell-Kenney, J. L. Jameson, and S. M. Moenter
Classical Estrogen Receptor {alpha} Signaling Mediates Negative and Positive Feedback on Gonadotropin-Releasing Hormone Neuron Firing
Endocrinology, November 1, 2008; 149(11): 5328 - 5334.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. H. Abel, P. J. Baker, H. M. Charlton, A. Monteiro, G. Verhoeven, K. De Gendt, F. Guillou, and P. J. O'Shaughnessy
Spermatogenesis and Sertoli Cell Activity in Mice Lacking Sertoli Cell Receptors for Follicle-Stimulating Hormone and Androgen
Endocrinology, July 1, 2008; 149(7): 3279 - 3285.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
S. Vuorenoja, A. Rivero-Muller, A. J Ziecik, I. Huhtaniemi, J. Toppari, and N. A Rahman
Targeted therapy for adrenocortical tumors in transgenic mice through their LH receptor by Hecate-human chorionic gonadotropin {beta} conjugate
Endocr. Relat. Cancer, June 1, 2008; 15(2): 635 - 648.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. T. Page, B. T. Marck, J. M. Tolliver, and A. M. Matsumoto
Tissue Selectivity of the Anabolic Steroid, 19-Nor-4-Androstenediol-3{beta},17{beta}-Diol in Male Sprague Dawley Rats: Selective Stimulation of Muscle Mass and Bone Mineral Density Relative to Prostate Mass
Endocrinology, April 1, 2008; 149(4): 1987 - 1993.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Bernichtein, E. Petretto, S. Jamieson, A. Goel, T. J. Aitman, J. M. Mangion, and I. T. Huhtaniemi
Adrenal Gland Tumorigenesis after Gonadectomy in Mice Is a Complex Genetic Trait Driven by Epistatic Loci
Endocrinology, February 1, 2008; 149(2): 651 - 661.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
J. Mittag, W. Oehr, H. Heuer, T. Hamalainen, B. Brachvogel, E. Poschl, and K. Bauer
Expression and thyroid hormone regulation of annexins in the anterior pituitary
J. Endocrinol., December 1, 2007; 195(3): 385 - 392.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
T. Saloniemi, T. Lamminen, K. Huhtinen, M. Welsh, P. Saunders, H. Kujari, and M. Poutanen
Activation of Androgens by Hydroxysteroid (17{beta}) Dehydrogenase 1 in Vivo as a Cause of Prenatal Masculinization and Ovarian Benign Serous Cystadenomas
Mol. Endocrinol., November 1, 2007; 21(11): 2627 - 2636.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
L. Faulkner, D. M. Altmann, S. Ellmerich, I. Huhtaniemi, G. Stamp, and S. Sriskandan
Sexual Dimorphism in Superantigen Shock Involves Elevated TNF-{alpha} and TNF-{alpha} induced Hepatic Apoptosis
Am. J. Respir. Crit. Care Med., September 1, 2007; 176(5): 473 - 482.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Zhongyi, P. Rantakari, T. Lamminen, J. Toppari, and M. Poutanen
Transgenic Male Mice Expressing Human Hydroxysteroid Dehydrogenase 2 Indicate a Role for the Enzyme Independent of Its Action on Sex Steroids
Endocrinology, August 1, 2007; 148(8): 3827 - 3836.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. M. Walden, T. D. Butters, R. A. Dwek, F. M. Platt, and A. C. van der Spoel
Long-term non-hormonal male contraception in mice using N-butyldeoxynojirimycin
Hum. Reprod., May 1, 2006; 21(5): 1309 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. Pielecka and S. M. Moenter
Effect of Steroid Milieu on Gonadotropin-Releasing Hormone-1 Neuron Firing Pattern and Luteinizing Hormone Levels in Male Mice
Biol Reprod, May 1, 2006; 74(5): 931 - 937.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
X. Li, L. Strauss, A. Kaatrasalo, A. Mayerhofer, I. Huhtaniemi, R. Santti, S. Makela, and M. Poutanen
Transgenic Mice Expressing P450 Aromatase as a Model for Male Infertility Associated with Chronic Inflammation in the Testis
Endocrinology, March 1, 2006; 147(3): 1271 - 1277.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
G. Shetty, C. C. Y. Weng, S. J. Meachem, O. U. Bolden-Tiller, Z. Zhang, P. Pakarinen, I. Huhtaniemi, and M. L. Meistrich
Both Testosterone and Follicle-Stimulating Hormone Independently Inhibit Spermatogonial Differentiation in Irradiated Rats
Endocrinology, January 1, 2006; 147(1): 472 - 482.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. A. Christian, J. L. Mobley, and S. M. Moenter
Diurnal and estradiol-dependent changes in gonadotropin-releasing hormone neuron firing activity
PNAS, October 25, 2005; 102(43): 15682 - 15687.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
T. Pakarainen, F.-P. Zhang, L. Nurmi, M. Poutanen, and I. Huhtaniemi
Knockout of Luteinizing Hormone Receptor Abolishes the Effects of Follicle-Stimulating Hormone on Preovulatory Maturation and Ovulation of Mouse Graafian Follicles
Mol. Endocrinol., October 1, 2005; 19(10): 2591 - 2602.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Bielinska, E. Genova, I. Boime, H. Parviainen, S. Kiiveri, J. Leppaluoto, N. Rahman, M. Heikinheimo, and D. B. Wilson
Gonadotropin-Induced Adrenocortical Neoplasia in NU/J Nude Mice
Endocrinology, September 1, 2005; 146(9): 3975 - 3984.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
H Santti, L Mikkonen, A Anand, S Hirvonen-Santti, J Toppari, M Panhuysen, F Vauti, M Perera, G Corte, W Wurst, et al.
Disruption of the murine PIASx gene results in reduced testis weight
J. Mol. Endocrinol., June 1, 2005; 34(3): 645 - 654.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
S. J Meachem, D. M Robertson, N. G Wreford, R. I McLachlan, and P. G Stanton
Oestrogen does not affect the restoration of spermatogenesis in the gonadotrophin-releasing hormone-immunised adult rat
J. Endocrinol., June 1, 2005; 185(3): 529 - 538.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. M. Robertson, G. U. Schuster, K. R. Steffensen, O. Hovatta, S. Meaney, K. Hultenby, L. C. Johansson, K. Svechnikov, O. Soder, and J.-A. Gustafsson
The Liver X Receptor-{beta} Is Essential for Maintaining Cholesterol Homeostasis in the Testis
Endocrinology, June 1, 2005; 146(6): 2519 - 2530.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
A. M. Vinggaard, S. Christiansen, P. Laier, M. E. Poulsen, V. Breinholt, K. Jarfelt, H. Jacobsen, M. Dalgaard, C. Nellemann, and U. Hass
Perinatal Exposure to the Fungicide Prochloraz Feminizes the Male Rat Offspring
Toxicol. Sci., June 1, 2005; 85(2): 886 - 897.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
T. E. Stoker, S. D. Perreault, K. Bremser, R. S. Marshall, A. Murr, and R. L. Cooper
Acute Exposure to Molinate Alters Neuroendocrine Control of Ovulation in the Rat
Toxicol. Sci., March 1, 2005; 84(1): 38 - 48.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Pakarainen, F.-P. Zhang, S. Makela, M. Poutanen, and I. Huhtaniemi
Testosterone Replacement Therapy Induces Spermatogenesis and Partially Restores Fertility in Luteinizing Hormone Receptor Knockout Mice
Endocrinology, February 1, 2005; 146(2): 596 - 606.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Jimenez, J.A. Spaliviero, A.J. Grootenhuis, J. Verhagen, C.M. Allan, and D.J. Handelsman
Validation of an Ultrasensitive and Specific Immunofluorometric Assay for Mouse Follicle-Stimulating Hormone
Biol Reprod, January 1, 2005; 72(1): 78 - 85.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Ahtiainen, J. Toppari, M. Poutanen, and I. Huhtaniemi
Indirect Sertoli Cell-Mediated Ablation of Germ Cells in Mice Expressing the Inhibin-{alpha} Promoter/Herpes Simplex Virus Thymidine Kinase Transgene
Biol Reprod, November 1, 2004; 71(5): 1545 - 1550.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
G. Shetty, C. C. Y. Weng, O. U. Bolden-Tiller, I. Huhtaniemi, D. J. Handelsman, and M. L. Meistrich
Effects of Medroxyprogesterone and Estradiol on the Recovery of Spermatogenesis in Irradiated Rats
Endocrinology, October 1, 2004; 145(10): 4461 - 4469.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
M. Zaleska, A. Waclawik, G. Bodek, A. Zezula-Szpyra, X. Li, T. Janowski, W. H. Hansel, N. A. Rahman, and A. J. Ziecik
Growth Repression in Diethylstilbestrol/Dimethylbenz[a]anthracene-Induced Rat Mammary Gland Tumor Using Hecate-CG{beta} Conjugate
Experimental Biology and Medicine, April 1, 2004; 229(4): 335 - 344.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
X. Li, L. Strauss, S. Makela, T. Streng, I. Huhtaniemi, R. Santti, and M. Poutanen
Multiple Structural and Functional Abnormalities in the P450 Aromatase Expressing Transgenic Male Mice Are Ameliorated by a P450 Aromatase Inhibitor
Am. J. Pathol., March 1, 2004; 164(3): 1039 - 1048.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
G. Shetty and C. C. Y. Weng
Cryptorchidism Rescues Spermatogonial Differentiation in Juvenile Spermatogonial Depletion (Jsd) Mice
Endocrinology, January 1, 2004; 145(1): 126 - 133.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
S. R. Bielmeier, D. S. Best, and M. G. Narotsky
Serum Hormone Characterization and Exogeneous Hormone Rescue of Bromodichloromethane-Induced Pregnancy Loss in the F344 Rat
Toxicol. Sci., January 1, 2004; 77(1): 101 - 108.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
M. L. Meistrich, G. Wilson, K. L. Porter, I. Huhtaniemi, G. Shetty, and G. A. Shuttlesworth
Restoration of Spermatogenesis in Dibromochloropropane (DBCP)-Treated Rats by Hormone Suppression
Toxicol. Sci., December 1, 2003; 76(2): 418 - 426.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F.-P. Zhang, T. Pakarainen, M. Poutanen, J. Toppari, and I. Huhtaniemi
The low gonadotropin-independent constitutive production of testicular testosterone is sufficient to maintain spermatogenesis
PNAS, November 11, 2003; 100(23): 13692 - 13697.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Bielinska, H. Parviainen, S. B. Porter-Tinge, S. Kiiveri, E. Genova, N. Rahman, I. T. Huhtaniemi, L. J. Muglia, M. Heikinheimo, and D. B. Wilson
Mouse Strain Susceptibility to Gonadectomy-Induced Adrenocortical Tumor Formation Correlates with the Expression of GATA-4 and Luteinizing Hormone Receptor
Endocrinology, September 1, 2003; 144(9): 4123 - 4133.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y. Lue, A. P. Sinha Hikim, C. Wang, A. Leung, and R. S. Swerdloff
Functional Role of Inducible Nitric Oxide Synthase in the Induction of Male Germ Cell Apoptosis, Regulation of Sperm Number, and Determination of Testes Size: Evidence from Null Mutant Mice
Endocrinology, July 1, 2003; 144(7): 3092 - 3100.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. Killian, K. Pratis, R. J. Clifton, P. G. Stanton, D. M. Robertson, and L. O'Donnell
5{alpha}-Reductase Isoenzymes 1 and 2 in the Rat Testis During Postnatal Development
Biol Reprod, May 1, 2003; 68(5): 1711 - 1718.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
C. Nellemann, M. Dalgaard, H. R. Lam, and A. M. Vinggaard
The Combined Effects of Vinclozolin and Procymidone Do Not Deviate from Expected Additivity in Vitro and in Vivo
Toxicol. Sci., February 1, 2003; 71(2): 251 - 262.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. J. Baker, P. Pakarinen, I. T. Huhtaniemi, M. H. Abel, H. M. Charlton, T. R. Kumar, and P. J. O'Shaughnessy
Failure of Normal Leydig Cell Development in Follicle-Stimulating Hormone (FSH) Receptor-Deficient Mice, But Not FSH{beta}-Deficient Mice: Role for Constitutive FSH Receptor Activity
Endocrinology, January 1, 2003; 144(1): 138 - 145.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. C. van der Spoel, M. Jeyakumar, T. D. Butters, H. M. Charlton, H. D. Moore, R. A. Dwek, and F. M. Platt
Reversible infertility in male mice after oral administration of alkylated imino sugars: A nonhormonal approach to male contraception
PNAS, December 24, 2002; 99(26): 17173 - 17178.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
P. Sipila, T. G. Cooper, C.-H. Yeung, M. Mustonen, J. Penttinen, J. Drevet, I. Huhtaniemi, and M. Poutanen
Epididymal Dysfunction Initiated by the Expression of Simian Virus 40 T-Antigen Leads to Angulated Sperm Flagella and Infertility in Transgenic Mice
Mol. Endocrinol., November 1, 2002; 16(11): 2603 - 2617.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
A. M. Vinggaard, C. Nellemann, M. Dalgaard, E. B. Jorgensen, and H. R. Andersen
Antiandrogenic Effects in Vitro and in Vivo of the Fungicide Prochloraz
Toxicol. Sci., October 1, 2002; 69(2): 344 - 353.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
R. A. DeFazio and S. M. Moenter
Estradiol Feedback Alters Potassium Currents and Firing Properties of Gonadotropin-Releasing Hormone Neurons
Mol. Endocrinol., October 1, 2002; 16(10): 2255 - 2265.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
G. Shetty, G. Wilson, M. P. Hardy, E. Niu, I. Huhtaniemi, and M. L. Meistrich
Inhibition of Recovery of Spermatogenesis in Irradiated Rats by Different Androgens
Endocrinology, September 1, 2002; 143(9): 3385 - 3396.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. S. Nunemaker, R. A. DeFazio, and S. M. Moenter
Estradiol-Sensitive Afferents Modulate Long-Term Episodic Firing Patterns of GnRH Neurons
Endocrinology, June 1, 2002; 143(6): 2284 - 2292.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
G. Shetty, G. Wilson, I. Huhtaniemi, H. Boettger-Tong, and M. L. Meistrich
Testosterone Inhibits Spermatogonial Differentiation in Juvenile Spermatogonial Depletion Mice
Endocrinology, July 1, 2001; 142(7): 2789 - 2795.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
X. Li, E. Nokkala, W. Yan, T. Streng, N. Saarinen, A. Warri, I. Huhtaniemi, R. Santti, S. Makela, and M. Poutanen
Altered Structure and Function of Reproductive Organs in Transgenic Male Mice Overexpressing Human Aromatase
Endocrinology, June 1, 2001; 142(6): 2435 - 2442.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y. Lue, P. N. Rao, A. P. Sinha Hikim, M. Im, W. A. Salameh, P. H. Yen, C. Wang, and R. S. Swerdloff
XXY Male Mice: An Experimental Model for Klinefelter Syndrome
Endocrinology, April 1, 2001; 142(4): 1461 - 1470.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
N. A. Rahman and I. T. Huhtaniemi
Ovarian Tumorigenesis in Mice Transgenic for Murine Inhibin {{alpha}} Subunit Promoter-Driven Simian Virus 40 T-Antigen: Ontogeny, Functional Characteristics, and Endocrine Effects
Biol Reprod, April 1, 2001; 64(4): 1122 - 1130.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
F.-P. Zhang, M. Poutanen, J. Wilbertz, and I. Huhtaniemi
Normal Prenatal but Arrested Postnatal Sexual Development of Luteinizing Hormone Receptor Knockout (LuRKO) Mice
Mol. Endocrinol., January 1, 2001; 15(1): 172 - 183.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
L.-J. Zhu, M. P. Hardy, I. V. Inigo, I. Huhtaniemi, C. W. Bardin, and A. J. Moo-Young
Effects of Androgen on Androgen Receptor Expression in Rat Testicular and Epididymal Cells: A Quantitative Immunohistochemical Study
Biol Reprod, August 1, 2000; 63(2): 368 - 376.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
G. Shetty, G. Wilson, I. Huhtaniemi, G. A. Shuttlesworth, T. Reissmann, and M. L. Meistrich
Gonadotropin-Releasing Hormone Analogs Stimulate and Testosterone Inhibits the Recovery of Spermatogenesis in Irradiated Rats
Endocrinology, May 1, 2000; 141(5): 1735 - 1745.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. H. Abel, A. N. Wootton, V. Wilkins, I. Huhtaniemi, P. G. Knight, and H. M. Charlton
The Effect of a Null Mutation in the Follicle-Stimulating Hormone Receptor Gene on Mouse Reproduction
Endocrinology, May 1, 2000; 141(5): 1795 - 1803.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y. Lue, A. P. Sinha Hikim, C. Wang, M. Im, A. Leung, and R. S. Swerdloff
Testicular Heat Exposure Enhances the Suppression of Spermatogenesis by Testosterone in Rats: The "Two-Hit" Approach to Male Contraceptive Development
Endocrinology, April 1, 2000; 141(4): 1414 - 1424.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J.I.J. van Casteren, W.G.E.J. Schoonen, and H.J. Kloosterboer
Development of Time-Resolved Immunofluorometric Assays for Rat Follicle-Stimulating Hormone and Luteinizing Hormone and Application on Sera of Cycling Rats
Biol Reprod, April 1, 2000; 62(4): 886 - 894.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
G. A. Shuttlesworth, D. G. De Rooij, I. Huhtaniemi, T. Reissmann, L. D. Russell, G. Shetty, G. Wilson, and M. L. Meistrich
Enhancement of A Spermatogonial Proliferation and Differentiation in Irradiated Rats by Gonadotropin-Releasing Hormone Antagonist Administration
Endocrinology, January 1, 2000; 141(1): 37 - 49.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Tena-Sempere, J. Kero, A. Rannikko, W. Yan, and I. Huhtaniemi
The Pattern of Inhibin/Activin {alpha}- and {beta}B-Subunit Messenger Ribonucleic Acid Expression in Rat Testis after Selective Leydig Cell Destruction by Ethylene Dimethane Sulfonate
Endocrinology, December 1, 1999; 140(12): 5761 - 5770.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
Y.-H. Lue, A. P. Sinha Hikim, R. S. Swerdloff, P. Im, K. S. Taing, T. Bui, A. Leung, and C. Wang
Single Exposure to Heat Induces Stage-Specific Germ Cell Apoptosis in Rats: Role of Intratesticular Testosterone on Stage Specificity
Endocrinology, April 1, 1999; 140(4): 1709 - 1717.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. D. Pierroz, A. C. Aebi, I. T. Huhtaniemi, and M. L. Aubert
Many LH peaks are needed to physiologically stimulate testosterone secretion: modulation by fasting and NPY
Am J Physiol Endocrinol Metab, April 1, 1999; 276(4): E603 - E610.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K. Hakola, D. D. Pierroz, A. Aebi, B. A.M. Vuagnat, M. L. Aubert, and I. Huhtaniemi
Dose and Time Relationships of Intravenously Injected Rat Recombinant Luteinizing Hormone and Testicular Testosterone Secretion in the Male Rat
Biol Reprod, August 1, 1998; 59(2): 338 - 343.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
P. J. O'Shaughnessy, P. Baker, U. Sohnius, A.-M. Haavisto, H. M. Charlton, and I. Huhtaniemi
Fetal Development of Leydig Cell Activity in the Mouse Is Independent of Pituitary Gonadotroph Function
Endocrinology, March 1, 1998; 139(3): 1141 - 1146.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Racine, R. Rey, M. G. Forest, F. Louis, A. Ferre, I. Huhtaniemi, N. Josso, and N. di Clemente
Receptors for anti-Mullerian hormone on Leydig cells are responsible for its effects on steroidogenesis and cell differentiation
PNAS, January 20, 1998; 95(2): 594 - 599.
[Abstract] [Full Text] [PDF]