| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Endocrinology, Vol 131, 799-806, Copyright © 1992 by Endocrine Society
ARTICLES |
JL Tilly, T Aihara, K Nishimori, XC Jia, H Billig, KI Kowalski, EA Perlas and AJ Hsueh
Department of Gynecology and Obstetrics, Stanford University School of Medicine, California 94305-5317.
The ligand specificity and biochemical properties of the human (h) FSH receptor are poorly characterized due to the low abundance of these receptors and the limited availability of human tissues. Using a fragment of rat FSH receptor cDNA, we screened a human testicular cDNA library and obtained a FSH receptor cDNA covering the entire amino acid- coding region. After transfection of a human fetal kidney cell line (293) with the hFSH receptor cDNA, radioligand receptor analysis revealed the presence of high affinity (Kd, 1.7 x 10(-9) M) FSH-binding sites on the plasma membrane. Both recombinant and wild-type hFSH displaced [125I]hFSH binding, with ED50 values of 25 and 70 ng/ml, respectively, whereas hLH, hCG, and hTSH were ineffective. Although human, rat(r), and ovine FSH as well as equine CG competed for rat testicular FSH receptor binding, only hFSH and rFSH interacted effectively with the recombinant hFSH receptor, suggesting that species- specific ligand recognition exists between human and rodent receptors. After incubation of transfected cells with hFSH, but not recombinant hLH or hCG, a dose-dependent increase (ED50, 10 ng/ml) in extracellular cAMP accumulation was observed, indicating a functional coupling of the expressed human receptor with the endogenous adenyl cyclase. In cells cotransfected with the FSH receptor expression plasmid and a luciferase reporter gene driven by the promoter of a cAMP-responsive gene, treatment with hFSH, but not hCG, resulted in a dose-dependent increase in luciferase activity. Northern blot analysis using a cRNA probe derived from the human receptor cDNA indicated the presence of multiple FSH receptor mRNA transcripts (7.0, 4.2, and 2.5 kilobases) in RNA prepared from human follicular phase ovary, but not from human corpus luteum or placenta. Additionally, two FSH-binding sites of 76 and 112 kilodaltons were detected in transfected 293 cells after ligand/receptor cross-linking and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. These results demonstrate the expression of functional hFSH receptor with unique ligand specificity and provide new data on the biochemical properties of the human receptor at the mRNA and protein levels.
This article has been cited by other articles:
 |
|
 |
|
  A. Navarro, R. Torrejon, M. J. Bandez, J. M. Lopez-Cepero, and A. Boveris Mitochondrial function and mitochondria-induced apoptosis in an overstimulated rat ovarian cycle Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E1101 - E1109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-K. So, H.-F. Kwok, and W. Ge Zebrafish Gonadotropins and Their Receptors: II. Cloning and Characterization of Zebrafish Follicle-Stimulating Hormone and Luteinizing Hormone Subunits--Their Spatial-Temporal Expression Patterns and Receptor Specificity Biol Reprod, June 1, 2005; 72(6): 1382 - 1396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Ji, C. Lee, M. Jeoung, Y. Koo, G. A. Sievert, and T. H. Ji Trans-Activation of Mutant Follicle-Stimulating Hormone Receptors Selectively Generates Only One of Two Hormone Signals Mol. Endocrinol., April 1, 2004; 18(4): 968 - 978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Forges, P. Monnier-Barbarino, G.C. Faure, and M.C. Bene Autoimmunity and antigenic targets in ovarian pathology Hum. Reprod. Update, March 1, 2004; 10(2): 163 - 175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Perlman, B. van den Hazel, J. Christiansen, S. Gram-Nielsen, C. B. Jeppesen, K. V. Andersen, T. Halkier, S. Okkels, and H. T. Schambye Glycosylation of an N-Terminal Extension Prolongs the Half-Life and Increases the in Vivo Activity of Follicle Stimulating Hormone J. Clin. Endocrinol. Metab., July 1, 2003; 88(7): 3227 - 3235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sudo, M. Kudo, S.-i. Wada, O. Sato, A. J.W. Hsueh, and S. Fujimoto Genetic and functional analyses of polymorphisms in the human FSH receptor gene Mol. Hum. Reprod., October 1, 2002; 8(10): 893 - 899. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lee, I. Ji, K. Ryu, Y. Song, P. M. Conn, and T. H. Ji Two Defective Heterozygous Luteinizing Hormone Receptors Can Rescue Hormone Action J. Biol. Chem., May 3, 2002; 277(18): 15795 - 15800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Meduri, N. Charnaux, M.-A. Driancourt, L. Combettes, P. Granet, B. Vannier, H. Loosfelt, and E. Milgrom Follicle-Stimulating Hormone Receptors in Oocytes? J. Clin. Endocrinol. Metab., May 1, 2002; 87(5): 2266 - 2276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Anderiesz, A.-P. Ferraretti, C. Magli, A. Fiorentino, D. Fortini, L. Gianaroli, G.M. Jones, and A.O. Trounson Effect of recombinant human gonadotrophins on human, bovine and murine oocyte meiosis, fertilization and embryonic development in vitro Hum. Reprod., May 1, 2000; 15(5): 1140 - 1148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Rose, R. E. Gaines Das, and A. H. Balen Definition and Measurement of Follicle Stimulating Hormone Endocr. Rev., February 1, 2000; 21(1): 5 - 22. [Abstract] [Full Text]
|
|
|
|
|
|
S. You, H. Kim, M. E. El Halawani, and D. N. Foster Three Different Turkey Luteinizing Hormone Receptor (tLH-R) Isoforms II: Characterization of Differentially Regulated tLH-R Messenger Ribonucleic Acid Isoforms in the Ovary Biol Reprod, January 1, 2000; 62(1): 117 - 124. [Abstract] [Full Text]
|
|
|
|
|
|
M. Tena-Sempere, P. R. Manna, and I. Huhtaniemi Molecular Cloning of the Mouse Follicle-Stimulating Hormone Receptor Complementary Deoxyribonucleic Acid: Functional Expression of Alternatively Spliced Variants and Receptor Inactivation by a C566T Transition in Exon 7 of the Coding Sequence Biol Reprod, June 1, 1999; 60(6): 1515 - 1527. [Abstract] [Full Text]
|
|
|
|
|
|
U.A. Vitt,, H.J. Kloosterboer,, U.M. Rose,, J.W.M. Mulders,, P.S. Kiesel,, S. Bete,, and P.L. Nayudu Isoforms of Human Recombinant Follicle-Stimulating Hormone: Comparison of Effects on Murine Follicle Development In Vitro Biol Reprod, October 1, 1998; 59(4): 854 - 861. [Abstract] [Full Text]
|
|
|
|
|
|
M. Simoni, J. Gromoll, and E. Nieschlag The Follicle-Stimulating Hormone Receptor: Biochemistry, Molecular Biology, Physiology, and Pathophysiology Endocr. Rev., December 1, 1997; 18(6): 739 - 773. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Osuga, M. Hayashi, M. Kudo, M. Conti, B. Kobilka, and A. J. W. Hsueh Co-expression of Defective Luteinizing Hormone Receptor Fragments Partially Reconstitutes Ligand-induced Signal Generation J. Biol. Chem., October 3, 1997; 272(40): 25006 - 25012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Osuga, M. Kudo, A. Kaipia, B. Kobilka, and A. J. W. Hsueh Derivation of Functional Antagonists Using N-Terminal Extracellular Domain of Gonadotropin and Thyrotropin Receptors Mol. Endocrinol., October 1, 1997; 11(11): 1659 - 1668. [Abstract] [Full Text]
|
|
|
|
|
|
D. P. Davis, T. G. Rozell, X. Liu, and D. L. Segaloff The Six N-linked Carbohydrates of the Lutropin/Choriogonadotropin Receptor Are Not Absolutely Required for Correct Folding, Cell Surface Expression, Hormone Binding, or Signal Transduction Mol. Endocrinol., May 1, 1997; 11(5): 550 - 562. [Abstract] [Full Text]
|
|
|
|
|
|
A. Hoek, J. Schoemaker, and H. A. Drexhage Premature Ovarian Failure and Ovarian Autoimmunity Endocr. Rev., February 1, 1997; 18(1): 107 - 134. [Abstract] [Full Text]
|
|
|
|
|
|
M. Kudo, Y. Osuga, B. K. Kobilka, and A. J.W. Hsueh Transmembrane Regions V and VI of the Human Luteinizing Hormone Receptor Are Required for Constitutive Activation by a Mutation in the Third Intracellular Loop J. Biol. Chem., September 13, 1996; 271(37): 22470 - 22478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sugahara, A. Sato, M. Kudo, D. Ben-Menahem, M. R. Pixley, A. J. W. Hsueh, and I. Boime Expression of Biologically Active Fusion Genes Encoding the Common alpha Subunit and the Follicle-stimulating Hormone beta Subunit J. Biol. Chem., May 3, 1996; 271(18): 10445 - 10448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. Bolger, I. McPhee, and M. D. Houslay Alternative Splicing of cAMP-specific Phosphodiesterase mRNA Transcripts J. Biol. Chem., January 12, 1996; 271(2): 1065 - 1071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Ji and T. H. Ji Differential Roles of Exoloop 1 of the Human Follicle-stimulating Hormone Receptor in Hormone Binding and Receptor Activation J. Biol. Chem., July 7, 1995; 270(27): 15970 - 15973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. S. Song, I. Ji, J. Beauchamp, N. W. Isaacs, and T. H. Ji Hormone Interactions to Leu-rich Repeats in the Gonadotropin Receptors. I. ANALYSIS OF LEU-RICH REPEATS OF HUMAN LUTEINIZING HORMONE/CHORIONIC GONADOTROPIN RECEPTOR AND FOLLICLE-STIMULATING HORMONE RECEPTOR J. Biol. Chem., January 26, 2001; 276(5): 3426 - 3435. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
