Endocrinology, Vol 130, 109-114, Copyright © 1992 by Endocrine Society

ARTICLES

Differential adrenergic regulation of beta 1- and beta 3-adrenoreceptor messenger ribonucleic acids in adipose tissues

JG Granneman and KN Lahners
Department of Psychiatry, Wayne State University School of Medicine, Detroit, Michigan 48207.

The levels of beta 1- and beta 3-adrenoreceptor mRNAs in several rat tissues were determined simultaneously with a sensitive nuclease protection assay. The beta 1-receptor gene was expressed to varying degrees in most tissues examined. By contrast, high levels of beta 3- receptor mRNA were only found in brown and white adipose tissues, indicating that beta 3-receptor gene expression is essentially adipose tissue specific. Surgical sympathectomy of interscapular brown adipose tissue increased beta 3-receptor mRNA levels by 2.4-fold, but did not affect beta 1-receptor mRNA levels. Exposure of rats to 4 C, which increases sympathetic nerve stimulation of IBAT, reduced beta 3- receptor mRNA levels in intact tissue but did not affect the denervation-induced increase in beta 3-receptor mRNA. Acute treatment of rats with norepinephrine greatly reduced beta 3 mRNA levels in both white and brown adipose tissues, but did not alter beta 1-receptor mRNA levels. These results indicate that beta 1- and beta 3-receptor mRNAs are differentially regulated and that norepinephrine released from sympathetic nerves is an important inhibitory regulator of beta 3- receptor mRNA levels. Injections of the beta-receptor agonist isoproterenol and the beta 3-receptor agonist BRL 26830 each reduced beta 3-receptor mRNA in brown and white fat, whereas injections of glucagon reduced beta 3-receptor mRNA in brown fat only. These data indicate that while stimulation of beta 3-receptors is sufficient to down-regulate beta 3 mRNA, other receptors that stimulate adenylyl cyclase have the same effect. Finally, the agonist-induced down- regulation of beta 3-receptor mRNA was associated with a reduction in beta 3-receptor activation of adenylyl cyclase in white adipose tissue.

This article has been cited by other articles:

				X. Q. Xiao, S. M. Williams, B. E. Grayson, M. M. Glavas, M. A. Cowley, M. S. Smith, and K. L. Grove Excess Weight Gain during the Early Postnatal Period Is Associated with Permanent Reprogramming of Brown Adipose Tissue Adaptive Thermogenesis Endocrinology, September 1, 2007; 148(9): 4150 - 4159. [Abstract] [Full Text] [PDF]

				T. J. Bartness and C. K. Song Thematic review series: Adipocyte Biology. Sympathetic and sensory innervation of white adipose tissue J. Lipid Res., August 1, 2007; 48(8): 1655 - 1672. [Abstract] [Full Text] [PDF]

				E. Chernogubova, D. S. Hutchinson, J. Nedergaard, and T. Bengtsson {alpha}1- and {beta}1-Adrenoceptor Signaling Fully Compensates for {beta}3-Adrenoceptor Deficiency in Brown Adipocyte Norepinephrine-Stimulated Glucose Uptake Endocrinology, May 1, 2005; 146(5): 2271 - 2284. [Abstract] [Full Text] [PDF]

				X. X. YU, D. A. LEWIN, W. FORREST, and S. H. ADAMS Cold elicits the simultaneous induction of fatty acid synthesis and {beta}-oxidation in murine brown adipose tissue: prediction from differential gene expression and confirmation in vivo FASEB J, February 1, 2002; 16(2): 155 - 168. [Abstract] [Full Text] [PDF]

				F. Marion-Latard, I. De Glisezinski, F. Crampes, M. Berlan, J. Galitzky, H. Suljkovicova, D. Riviere, and V. Stich A single bout of exercise induces {beta}-adrenergic desensitization in human adipose tissue Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2001; 280(1): R166 - R173. [Abstract] [Full Text] [PDF]

				M. O. Ribeiro, F. L. A. S. Lebrun, M. A. Christoffolete, M. Branco, A. Crescenzi, S. D. Carvalho, N. Negrao, and A. C. Bianco Evidence of UCP1-independent regulation of norepinephrine-induced thermogenesis in brown fat Am J Physiol Endocrinol Metab, August 1, 2000; 279(2): E314 - E322. [Abstract] [Full Text] [PDF]

				E. Farias-Silva, D. M. Grassi-Kassisse, V. Wolf-Nunes, and R. C. Spadari-Bratfisch Stress-induced alteration in the lipolytic response to {beta}-adrenoceptor agonists in rat white adipocytes J. Lipid Res., September 1, 1999; 40(9): 1719 - 1727. [Abstract] [Full Text]

				T. Ikezu, S. Yasuhara, J. G. Granneman, F. B. Kraemer, T. Okamoto, R. G. Tompkins, and J. A. J. Martyn A unique mechanism of desensitization to lipolysis mediated by beta 3-adrenoceptor in rats with thermal injury Am J Physiol Endocrinol Metab, August 1, 1999; 277(2): E316 - E324. [Abstract] [Full Text] [PDF]

				A. Chaudhry and J. G. Granneman Differential regulation of functional responses by beta -adrenergic receptor subtypes in brown adipocytes Am J Physiol Regulatory Integrative Comp Physiol, July 1, 1999; 277(1): R147 - R153. [Abstract] [Full Text] [PDF]

				J. Zhao, B. Cannon, and J. Nedergaard Thermogenesis is beta 3- but not beta 1-adrenergically mediated in rat brown fat cells, even after cold acclimation Am J Physiol Regulatory Integrative Comp Physiol, December 1, 1998; 275(6): R2002 - R2011. [Abstract] [Full Text] [PDF]

				A. Dicker, J. Zhao, B. Cannon, and J. Nedergaard Apparent thermogenic effect of injected glucagon is not due to a direct effect on brown fat cells Am J Physiol Regulatory Integrative Comp Physiol, November 1, 1998; 275(5): R1674 - R1682. [Abstract] [Full Text] [PDF]

				P. J. Scarpace and M. Matheny Leptin induction of UCP1 gene expression is dependent on sympathetic innervation Am J Physiol Endocrinol Metab, August 1, 1998; 275(2): E259 - E264. [Abstract] [Full Text] [PDF]

				J.-L. Lécrivain, J. Cohen-Tannoudji, M.-T. Robin, N. Coudouel, C. Legrand, and J.-P. Maltier Molecular Mechanisms of Adenylyl Cyclase Desensitization in Pregnant Rat Myometrium following In Vivo Administration of the ss-Adrenergic Agonist, Isoproterenol Biol Reprod, July 1, 1998; 59(1): 45 - 52. [Abstract] [Full Text]

				J. G. Granneman, Y. Zhai, and K. N. Lahners Selective Up-Regulation of alpha 1a-Adrenergic Receptor Protein and mRNA in Brown Adipose Tissue by Neural and beta 3-Adrenergic Stimulation Mol. Pharmacol., April 1, 1997; 51(4): 644 - 650. [Abstract] [Full Text]

				V Zilberfarb, F Pietri-Rouxel, R Jockers, S Krief, C Delouis, T Issad, and A. Strosberg Human immortalized brown adipocytes express functional beta3-adrenoceptor coupled to lipolysis J. Cell Sci., January 4, 1997; 110(7): 801 - 807. [Abstract] [PDF]

				S. Collins, K. W. Daniel, A. E. Petro, and R. S. Surwit Strain-Specific Response to {beta}3-Adrenergic Receptor Agonist Treatment of Diet-Induced Obesity in Mice Endocrinology, January 1, 1997; 138(1): 405 - 413. [Abstract] [Full Text] [PDF]

				T. Bengtsson, K. Redegren, A. D. Strosberg, J. Nedergaard, and B. Cannon Down-regulation of beta 3 Adrenoreceptor Gene Expression in Brown Fat Cells Is Transient and Recovery Is Dependent upon a Short-lived Protein Factor J. Biol. Chem., December 27, 1996; 271(52): 33366 - 33375. [Abstract] [Full Text] [PDF]

				B. G. Tholanikunnel, J. G. Granneman, and C. C. Malbon The M[IMAGE] 35,000 [IMAGE]-Adrenergic Receptor mRNA-binding Protein Binds Transcripts of G-protein-linked Receptors Which Undergo Agonist-induced Destabilization J. Biol. Chem., May 26, 1995; 270(21): 12787 - 12793. [Abstract] [Full Text] [PDF]

				J. Wang and E. M. Ross The Carboxyl-terminal Anchorage Domain of the Turkey beta(1)-Adrenergic Receptor Is Encoded by an Alternatively Spliced Exon J. Biol. Chem., March 24, 1995; 270(12): 6488 - 6495. [Abstract] [Full Text] [PDF]