Endocrinology, Vol 129, 2862-2866, Copyright © 1991 by Endocrine Society

ARTICLES

Interleukin-1 beta induces interleukin-1 alpha messenger ribonucleic acid expression in primary cultures of Leydig cells

DL Wang, ML Nagpal, JH Calkins, WW Chang, MM Sigel and T Lin
Medical Service, W. J. B. Dorn Veterans Hospital, Columbia, South Carolina 29201.

Previously, we have reported that interleukin-1 (IL-1) can modulate Leydig cell steroidogenesis. Recently, IL-1-like material has been shown to be present in the testis; however, the cellular source of this material remains unclear. In the present study we found that human recombinant IL-1 beta (1-100 ng/ml) caused dose-dependent increases in IL-1 alpha mRNA expression in Leydig cells. Similar to that reported in other tissues, IL-1 alpha mRNA from Leydig cells is mainly 2.2 kilobases. IL-1 alpha mRNA expression in Leydig cells was detectable as early as 2 h after the addition of IL-1 beta (10 ng/ml) and persisted for up to 24 h. Lipopolysaccharide also stimulated IL-1 alpha mRNA expression in these cells, but phorbol ester had no effect. Our results indicate that Leydig cells are a potential source of IL-1, which has both autocrine and paracrine effects.

This article has been cited by other articles:

				M. K. O'Bryan, O. Gerdprasert, D. J. Nikolic-Paterson, A. Meinhardt, J. A. Muir, L. M. Foulds, D. J. Phillips, D. M. de Kretser, and M. P. Hedger Cytokine profiles in the testes of rats treated with lipopolysaccharide reveal localized suppression of inflammatory responses Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1744 - R1755. [Abstract] [Full Text] [PDF]

				L. Walch and P. L. Morris Cyclooxygenase 2 Pathway Mediates IL-1{beta} Regulation of IL-1{alpha}, -1{beta}, and IL-6 mRNA Levels in Leydig Cell Progenitors Endocrinology, September 1, 2002; 143(9): 3276 - 3283. [Abstract] [Full Text] [PDF]

				D. Zeyse, E. Lunenfeld, M. Beck, I. Prinsloo, and M. Huleihel Induction of Interleukin-1{alpha} Production in Murine Sertoli Cells by Interleukin-1 Biol Reprod, May 1, 2000; 62(5): 1291 - 1296. [Abstract] [Full Text]

				D. Zeyse, E. Lunenfeld, M. Beck, I. Prinsloo, and M. Huleihel Interleukin-1 Receptor Antagonist Is Produced by Sertoli Cells in Vitro Endocrinology, April 1, 2000; 141(4): 1521 - 1527. [Abstract] [Full Text] [PDF]

				J. Hu, S. You, W. Li, D. Wang, M. L. Nagpal, Y. Mi, P. Liang, and T. Lin Expression and Regulation of Interferon-{gamma}-Inducible Protein 10 Gene in Rat Leydig Cells Endocrinology, August 1, 1998; 139(8): 3637 - 3645. [Abstract] [Full Text] [PDF]

				L. Gnessi, A. Fabbri, and G. Spera Gonadal Peptides as Mediators of Development and Functional Control of the Testis: An Integrated System with Hormones and Local Environment Endocr. Rev., August 1, 1997; 18(4): 541 - 609. [Abstract] [Full Text] [PDF]

				C. Cudicini, H. Lejeune, E. Gomez, E. Bosmans, F. Ballet, J. Saez, and B. Jegou Human Leydig Cells and Sertoli Cells Are Producers of Interleukins-1 and -6 J. Clin. Endocrinol. Metab., May 1, 1997; 82(5): 1426 - 1433. [Abstract] [Full Text] [PDF]