Endocrinology, Vol 129, 2225-2230, Copyright © 1991 by Endocrine Society

ARTICLES

Intracerebroventricular atrial natriuretic factor (ANF) antiserum inhibits volume-induced ANF in sheep: evidence for the brain's regulation of ANF secretion

CJ Charles, F Tang, VA Cameron, AM Richards and EA Espiner
Department of Endocrinology, Princess Margaret Hospital, Christchurch, New Zealand.

Plasma volume expansion stimulates cardiac secretion of atrial natriuretic factor (ANF) and also increases the ANF concentration in cerebrospinal fluid. In order to determine whether brain ANF is involved in the compensatory response to hypervolemia or the regulation of cardiac secretion of ANF, we have studied the integrated hemodynamic, renal, and hormonal response to acute volume expansion (15 ml/kg Dextran over 30 min) in five sheep given nonimmune serum (control) and ANF antiserum by intracerebroventricular (icv) injections on separate days. Dextran loading caused similar decreases in hematocrit and increases in central venous and mean arterial pressures on both study days. Heart rate was higher after antiserum injections (P less than 0.05). Dextran loading increased plasma ANF on the control (20 pmol/liter maximal mean increment above baseline) but not on the antiserum day (P less than 0.01). The diuresis (P less than 0.01) and natriuresis (P less than 0.05) observed on the control day was inhibited by icv antiserum. Plasma aldosterone and cortisol levels showed similar falls in response to the dextran load on both days. These experiments show that icv ANF antiserum inhibits both the increase in cardiac secretion of ANF and the renal response to plasma volume expansion without affecting hemodynamic status. These data support the hypothesis that the brain ANF system is important in the systemic responses to volume loading.

This article has been cited by other articles:

				J. ANTUNES-RODRIGUES, M. DE CASTRO, L. L. K. ELIAS, M. M. VALENCA, and S. M. McCANN Neuroendocrine Control of Body Fluid Metabolism Physiol Rev, January 1, 2004; 84(1): 169 - 208. [Abstract] [Full Text] [PDF]

				C. J. Charles, M. T. Rademaker, A. M. Richards, G. J. S. Cooper, D. H. Coy, and M. G. Nicholls Hemodynamic, Hormonal, and Renal Effects of Intracerebroventricular Adrenomedullin in Conscious Sheep Endocrinology, April 1, 1998; 139(4): 1746 - 1751. [Abstract] [Full Text] [PDF]

				A. V. Turnbull and C. L. Rivier Intracerebroventricular Passive Immunization. II. Intracerebroventricular Infusion of Neuropeptide Antisera Can Inhibit Neuropeptide Signaling in Peripheral Tissues Endocrinology, January 1, 1998; 139(1): 128 - 136. [Abstract] [Full Text] [PDF]