Endocrinology, Vol 129, 1621-1627, Copyright © 1991 by Endocrine Society

ARTICLES

Luteinizing hormone induces progesterone receptor gene expression in cultured porcine granulosa cells

M Iwai, K Yasuda, M Fukuoka, T Iwai, K Takakura, S Taii, S Nakanishi and T Mori
Department of Gynecology & Obstetrics, Faculty of Medicine, Kyoto University, Japan.

We have examined the effect of LH on the regulation of the progesterone receptor (PR) in cultured porcine granulosa cells. In this study we used the RNase protection assay to evaluate the PR mRNA levels with a porcine cDNA clone isolated by the polymerase chain reaction (PCR) method. This clone was regarded as part of the porcine PR cDNA because of its 98.3% and 95.7% homology to the hormone-binding domain of human PR cDNA in amino acid and nucleotide sequences, respectively. Treatment with LH (500 ng/ml) increased porcine PR mRNA to a maximum level of 8.6 +/- 1.1-fold (mean +/- SE) after 3-h exposure. This induction was mimicked by (Bu)2cAMP as well as by FSH and hCG, and the increased PR caused by LH and (Bu)2cAMP occurred in a dose-dependent manner. Basal and LH-induced PR mRNA levels were not affected by progesterone (100 ng/ml), estrogen (100 ng/ml), and RU 486 (10 ng/ml) at 3 h. The mechanism of the increased PR mRNA levels was studied in the presence of actinomycin-D and cycloheximide. While inhibition of RNA synthesis with actinomycin-D blocked LH-induced PR mRNA expression, inhibition of protein synthesis with cycloheximide increased basal and LH-induced PR mRNA levels. These results indicate that the expression of PR mRNA is positively regulated by LH, and this induction does not require ongoing protein synthesis. There may be a cycloheximide-sensitive mechanism that modulates PR mRNA stability. From our results we suspect that progesterone modulates ovarian function through LH-induced PR in granulosa cells.

This article has been cited by other articles:

				S. M. Quirk, R. G. Cowan, and R. M. Harman Progesterone Receptor and the Cell Cycle Modulate Apoptosis in Granulosa Cells Endocrinology, November 1, 2004; 145(11): 5033 - 5043. [Abstract] [Full Text] [PDF]

				T. R. Bilby, A. Guzeloglu, S. Kamimura, S. M. Pancarci, F. Michel, H. H. Head, and W. W. Thatcher Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: I. Ovarian, Conceptus, and Insulin-Like Growth Factor System Responses J Dairy Sci, October 1, 2004; 87(10): 3256 - 3267. [Abstract] [Full Text] [PDF]

				A. Guzeloglu, T. R. Bilby, A. Meikle, S. Kamimura, A. Kowalski, F. Michel, L. A. MacLaren, and W. W. Thatcher Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy J Dairy Sci, October 1, 2004; 87(10): 3268 - 3279. [Abstract] [Full Text] [PDF]

				M. Jo, C.M. Komar, and J.E. Fortune Gonadotropin Surge Induces Two Separate Increases in Messenger RNA for Progesterone Receptor in Bovine Preovulatory Follicles Biol Reprod, December 1, 2002; 67(6): 1981 - 1988. [Abstract] [Full Text] [PDF]

				C.L. Swan, M.C. Agostini, P.M. Bartlewski, V. Feyles, R.J. Urban, and P.J. Chedrese Effects of Progestins on Progesterone Synthesis in a Stable Porcine Granulosa Cell Line: Control of Transcriptional Activity of the Cytochrome P450 Side-Chain Cleavage Gene Biol Reprod, April 1, 2002; 66(4): 959 - 965. [Abstract] [Full Text] [PDF]

				K. Takakura and A. M. Parfitt Author's Response: The Role of Human Chorionic Gonadotropin and cAMP/Protein Kinase A Signaling in the Differentiation of Human Endometrial Stromal Cells J. Clin. Endocrinol. Metab., August 1, 2001; 86(8): 4001 - 4001. [Full Text] [PDF]

				T. El-Hefnawy, P. R. Manna, M. Luconi, E. Baldi, J. P. Slotte, and I. Huhtaniemi Progesterone Action in a Murine Leydig Tumor Cell Line (mLTC-1), Possibly through a Nonclassical Receptor Type Endocrinology, January 1, 2000; 141(1): 247 - 255. [Abstract] [Full Text] [PDF]

				C. Ko, Y.-H. In, and O.-K. Park-Sarge Role of Progesterone Receptor Activation in Pituitary Adenylate Cyclase Activating Polypeptide Gene Expression in Rat Ovary Endocrinology, November 1, 1999; 140(11): 5185 - 5194. [Abstract] [Full Text]

				R. C. M. Simmen, T. E. Chung, H. Imataka, F. J. Michel, L. Badinga, and F. A. Simmen Trans-Activation Functions of the Sp-Related Nuclear Factor, Basic Transcription Element-Binding Protein, and Progesterone Receptor in Endometrial Epithelial Cells Endocrinology, June 1, 1999; 140(6): 2517 - 2525. [Abstract] [Full Text]

				D. M. Duffy, D. R. Stewart, and R. L. Stouffer Titrating Luteinizing Hormone Replacement to Sustain the Structure and Function of the Corpus Luteum after Gonadotropin-Releasing Hormone Antagonist Treatment in Rhesus Monkeys J. Clin. Endocrinol. Metab., January 1, 1999; 84(1): 342 - 349. [Abstract] [Full Text]

				S. Hild-Petito and A. T. Fazleabas Expression of Steroid Receptors and Steroidogenic Enzymes in the Baboon (Papio anubis) Corpus Luteum during the Menstrual Cycle and Early Pregnancy J. Clin. Endocrinol. Metab., March 1, 1997; 82(3): 955 - 962. [Abstract] [Full Text] [PDF]

				M. Byers, G. G. J. M. Kuiper, J.-A. Gustafsson, and O.-K. Park-Sarge Estrogen Receptor-{beta} mRNA Expression in Rat Ovary: Down-Regulation by Gonadotropins Mol. Endocrinol., February 1, 1997; 11(2): 172 - 182. [Abstract] [Full Text]