Endocrinology, Vol 128, 2976-2984, Copyright © 1991 by Endocrine Society

ARTICLES

Prolactin receptor gene expression in rat mammary gland and liver during pregnancy and lactation

GA Jahn, M Edery, L Belair, PA Kelly and J Djiane
Unite d'Endocrinologie Moleculaire, Institut National de la Recherche Aaronomique, Jouy-en-Josas, France.

The expression of two forms of PRL receptor messenger RNA was measured at different stages of pregnancy and lactation in mammary gland and liver from Sprague-Dawley rats, using 32P-labeled complementary DNA probes encoding the extracellular part of the receptor (E probe), common to the two forms and a probe encoding the intracellular part of the long form of the receptor (I probe), that only recognizes sequences specific to the long form of the receptor. Hybridizations were performed in Northern blots obtained from electrophoreses of poly (A+) enriched RNA preparations from mammary glands and livers of rats on days 0, 6, 12, 19, and 21 of pregnancy and 5, 10, 15, and 20 of lactation. The Northern blots were also hybridized with a chicken beta- actin probe, to correct for the amount of mRNA added and the different metabolic states of the tissues. Both tissues expressed the same forms of PRL receptor mRNAs, namely bands at 2.5, 3, and 5.5 kilobases encoding the long form of the receptor and a major band at 1.8 kilobases encoding the short form. The liver expressed all the receptor mRNA forms in much higher quantity than the mammary gland, independent of the reproductive state. In liver there was an increase of all the transcripts on day 19 of pregnancy, followed by an abrupt decline at the onset of lactation, to levels lower than those of virgin rats. In contrast, mammary gland PRL receptor mRNAs were low in virgin and pregnant animals, increased significantly at day 21 of pregnancy, and continued to increase throughout lactation. Treatment of day 19 pregnant rats with the antiprogesterone RU 486 induced, 24 h later, PRL receptor mRNAs in mammary gland but not in liver. There were no significant differences in the relative proportions of long to short forms of PRL receptor mRNAs at the different reproductive states, but the proportion of the long form was slightly greater in mammary gland than in liver. Membrane PRL receptor concentrations were also measured in the same tissues used for the mRNA study by binding to a 125I- labeled monoclonal antibody (U5), which specifically recognizes the PRL receptor at a site different from the hormone binding site. The quantity of receptor measured by U5 binding was approximately 3 times higher than that measured with 125I-labeled ovine PRL.(ABSTRACT TRUNCATED AT 400 WORDS)

This article has been cited by other articles:

				C. Deachapunya, S. Poonyachoti, and N. Krishnamra Regulation of electrolyte transport across cultured endometrial epithelial cells by prolactin J. Endocrinol., June 1, 2008; 197(3): 575 - 582. [Abstract] [Full Text] [PDF]

				N. Ben-Jonathan, C. R. LaPensee, and E. W. LaPensee What Can We Learn from Rodents about Prolactin in Humans? Endocr. Rev., February 1, 2008; 29(1): 1 - 41. [Abstract] [Full Text] [PDF]

				T. L. Auchtung, A. G. Rius, P. E. Kendall, T. B. McFadden, and G. E. Dahl Effects of Photoperiod During the Dry Period on Prolactin, Prolactin Receptor, and Milk Production of Dairy Cows J Dairy Sci, January 1, 2005; 88(1): 121 - 127. [Abstract] [Full Text] [PDF]

				M. Matasconi, B. Angelin, and M. Rudling Pituitary control of lipoprotein and bile acid metabolism in male rats: growth hormone effects are not mediated by prolactin Am J Physiol Endocrinol Metab, July 1, 2004; 287(1): E114 - E119. [Abstract] [Full Text] [PDF]

				J. E. B. Cavaco, C. R.A. Santos, P. M. Ingleton, A. V.M. Canario, and D. M. Power Quantification of Prolactin (PRL) and PRL Receptor Messenger RNA in Gilthead Seabream (Sparus aurata) after Treatment with Estradiol-17{beta} Biol Reprod, February 1, 2003; 68(2): 588 - 594. [Abstract] [Full Text] [PDF]

				M. Tanaka, Y. Hayashida, T. Iguchi, N. Nakao, M. Suzuki, N. Nakai, and K. Nakashima Identification of a Novel First Exon of Prolactin Receptor Gene Expressed in the Rat Brain Endocrinology, June 1, 2002; 143(6): 2080 - 2084. [Abstract] [Full Text] [PDF]

				C. Ling and H. Billig PRL Receptor-Mediated Effects in Female Mouse Adipocytes: PRL Induces Suppressors of Cytokine Signaling Expression and Suppresses Insulin-Induced Leptin Production in Adipocytes in Vitro Endocrinology, November 1, 2001; 142(11): 4880 - 4890. [Abstract] [Full Text] [PDF]

				S. P. Tam, P. Lau, J. Djiane, D. J. Hilton, and M. J. Waters Tissue-Specific Induction of SOCS Gene Expression by PRL Endocrinology, November 1, 2001; 142(11): 5015 - 5026. [Abstract] [Full Text] [PDF]

				J. Cao, P. M. Gowri, T. C. Ganguly, M. Wood, J. F. Hyde, F. Talamantes, and M. Vore PRL, Placental Lactogen, and GH Induce Na+/Taurocholate-Cotransporting Polypeptide Gene Expression by Activating Signal Transducer and Activator of Transcription-5 in Liver Cells Endocrinology, October 1, 2001; 142(10): 4212 - 4222. [Abstract] [Full Text] [PDF]

				C. Ling, G. Hellgren, M. Gebre-Medhin, K. Dillner, H. Wennbo, B. Carlsson, and H. Billig Prolactin (PRL) Receptor Gene Expression in Mouse Adipose Tissue: Increases during Lactation and in PRL-Transgenic Mice Endocrinology, October 1, 2000; 141(10): 3564 - 3572. [Abstract] [Full Text] [PDF]

				F. E. Jones, T. Welte, X.-Y. Fu, and D. F. Stern ErbB4 Signaling in the Mammary Gland Is Required for Lobuloalveolar Development and Stat5 Activation during Lactation J. Cell Biol., October 4, 1999; 147(1): 77 - 88. [Abstract] [Full Text] [PDF]

				J. N. C. Mao, J. Burnside, L. Li, J. Tang, C. Davolos, and L. A. Cogburn Characterization of Unique Truncated Prolactin Receptor Transcripts, Corresponding to the Intracellular Domain, in the Testis of the Sexually Mature Chicken Endocrinology, March 1, 1999; 140(3): 1165 - 1174. [Abstract] [Full Text]

				D. J. Tortonese, J. Brooks, P. M. Ingleton, and A. S. McNeilly Detection of Prolactin Receptor Gene Expression in the Sheep Pituitary Gland and Visualization of the Specific Translation of the Signal in Gonadotrophs Endocrinology, December 1, 1998; 139(12): 5215 - 5223. [Abstract] [Full Text] [PDF]

				H. N. Jabbour, H. O. D. Critchley, and S. C. Boddy Expression of Functional Prolactin Receptors in Nonpregnant Human Endometrium: Janus Kinase-2, Signal Transducer and Activator of Transcription-1 (STAT1), and STAT5 Proteins Are Phosphorylated after Stimulation with Prolactin J. Clin. Endocrinol. Metab., July 1, 1998; 83(7): 2545 - 2553. [Abstract] [Full Text]

				C. M. Telleria, T. G. Parmer, L. Zhong, D. L. Clarke, C. T. Albarracin, W. R. Duan, D. I. H. Linzer, and G. Gibori The Different Forms of the Prolactin Receptor in the Rat Corpus Luteum: Developmental Expression and Hormonal Regulation in Pregnancy Endocrinology, November 1, 1997; 138(11): 4812 - 4820. [Abstract] [Full Text] [PDF]

				L. A. Arbogast and J. L. Voogt Prolactin (PRL) Receptors Are Colocalized in Dopaminergic Neurons in Fetal Hypothalamic Cell Cultures: Effect of PRL on Tyrosine Hydroxylase Activity Endocrinology, July 1, 1997; 138(7): 3016 - 3023. [Abstract] [Full Text] [PDF]