Endocrinology, Vol 125, 1253-1259, Copyright © 1989 by Endocrine Society

ARTICLES

Variations in the culture medium for FRTL5 cells: effects on growth and iodide uptake

M Zakarija and JM McKenzie
Department of Medicine, University of Miami School of Medicine, Florida 33101.

We evaluated the composition of the medium in which FRTL5 cells were incubated for effects on basal and stimulated growth and differentiated function. Variables included the concentration of calf serum (CS) from 0% (0.2% BSA) to 5% and the addition of insulin or insulin-like growth factor-I (IGF-I); stimulation was by TSH, with some experiments using thyroid-stimulating antibody-immunoglobulin G. The basal rate of incorporation of [3H]thymidine and the content of DNA per well were enhanced progressively by the increasing concentration of CS, whether in a pretreatment period of 3 days or over a subsequent 2 days of incubation. Concomitantly, I- uptake was progressively inhibited. Stimulation of growth by TSH required serum and was greatest with 5% CS; TSH effect on I- uptake was progressively decreased by increments of CS. Insulin and IGF-I had effects comparable to each other, but much smaller concentrations of IGF-I were required; they both augmented growth and enhanced growth stimulation by TSH. These actions were progressively annulled by increasing concentrations of CS. I- uptake (basal and stimulated) was inhibited by insulin and IGF-I, but this inhibitory effect was abolished by CS, particularly at 5% concentrations. Overall, the influences of varying the concentrations of CS and the effects of insulin or IGF-I were consistent; if there was enhancement of growth stimulation, there was inhibition of the TSH effect on I- uptake. Underlying mechanisms for these observations have yet to be elucidated.

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