Endocrinology, Vol 124, 339-345, Copyright © 1989 by Endocrine Society

ARTICLES

Inhibitory effects of tumor necrosis factor-alpha and interferon-gamma on deoxyribonucleic acid and collagen synthesis by rat osteosarcoma cells (ROS 17/2.8)

MS Nanes, WM McKoy and SJ Marx
Mineral Metabolism Section, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, Maryland 20892.

Tumor necrosis factor-alpha (TNF alpha) and interferon-gamma (IFN gamma) have potent effects on bone resorption and collagen synthesis in cultured rat long bones. Since the effects of TNF alpha and IFN gamma may result from interaction with multiple cell types, we studied the effects of these cytokines on the synthesis of DNA and collagen in one cell type with osteoblast phenotype, cloned rat osteosarcoma cells (ROS 17/2.8). Recombinant human TNF alpha did not affect DNA synthesis after 48 h with concentrations of 10(-11)-10(-8) M and inhibited DNA synthesis slightly at 10(-6) M. Recombinant rat IFN gamma (5-500 U/ml) caused a dose-dependent inhibition of DNA synthesis. Coincubation with TNF alpha and IFN gamma inhibited DNA synthesis more than maximal doses of either cytokine alone. This enhanced inhibitory effect was due to the induction of a response to TNF alpha by IFN gamma, since preexposure of cells to IFN gamma for 24 h, followed by incubation with TNF alpha alone for an additional 48 h, also resulted in increased inhibition of DNA synthesis. Preexposure to TNF alpha for 24 h, followed by IFN gamma alone, did not increase the inhibition of DNA synthesis. Incubation with either IFN gamma (5-500 U/ml) or TNF alpha (10(-10)-10(-6) M) inhibited the incorporation of [3H]proline into collagen. Coincubation with intermediate concentrations of both cytokines resulted in an inhibitory effect greater than that produced by maximal concentrations of either alone. The results indicate that 1) IFN gamma and TNF alpha have direct actions on osteoblast-like cells in vitro; 2) IFN gamma modulates the DNA response to TNF alpha; and 3) the greater responses to combined cytokines than to high doses of either alone suggest that these cytokines act, at least in part, through different pathways.

This article has been cited by other articles:

				J. Lorenzo, M. Horowitz, and Y. Choi Osteoimmunology: Interactions of the Bone and Immune System Endocr. Rev., June 1, 2008; 29(4): 403 - 440. [Abstract] [Full Text] [PDF]

				L. Gilbert, X. He, P. Farmer, S. Boden, M. Kozlowski, J. Rubin, and M. S. Nanes Inhibition of Osteoblast Differentiation by Tumor Necrosis Factor-{alpha} Endocrinology, November 1, 2000; 141(11): 3956 - 3964. [Abstract] [Full Text] [PDF]

				Y. Azuma, K. Kaji, R. Katogi, S. Takeshita, and A. Kudo Tumor Necrosis Factor-alpha Induces Differentiation of and Bone Resorption by Osteoclasts J. Biol. Chem., February 18, 2000; 275(7): 4858 - 4864. [Abstract] [Full Text] [PDF]

				K. Kurokouchi, C. R. Jacobs, and H. J. Donahue Oscillating Fluid Flow Inhibits TNF-alpha -induced NF-kappa B Activation via an Ikappa B Kinase Pathway in Osteoblast-like UMR106 Cells J. Biol. Chem., April 13, 2001; 276(16): 13499 - 13504. [Abstract] [Full Text] [PDF]

				L. Gilbert, X. He, P. Farmer, J. Rubin, H. Drissi, A. J. van Wijnen, J. B. Lian, G. S. Stein, and M. S. Nanes Expression of the Osteoblast Differentiation Factor RUNX2 (Cbfa1/AML3/Pebp2alpha A) Is Inhibited by Tumor Necrosis Factor-alpha J. Biol. Chem., January 18, 2002; 277(4): 2695 - 2701. [Abstract] [Full Text] [PDF]