Endocrinology, Vol 122, 1182-1184, Copyright © 1988 by Endocrine Society

ARTICLES

Insulin stimulates protein synthesis in compacted mouse embryos

MB Harvey and PL Kaye
Department of Physiology and Pharmacology, University of Queensland, St. Lucia, Australia.

Preliminary work suggested that insulin affects preimplantation mouse embryos. We investigated the effects of insulin on incorporation of [3H]-leucine by embryos cultured for long (greater than 24 h) or short (4 h) terms with insulin. Insulin stimulated incorporation by compacted 8-cell embryos, blastocysts and expanded blastocysts. The stimulation in blastocysts had an EC50 of 0.5 pM in the presence of 1 g/L BSA and was blocked by anti-human insulin anti-serum. Inclusion of insulin in the medium elevated the levels of incorporation towards those of embryos developing in vivo. The stimulation was not observed in uncompacted 8-cell embryos. The ontogeny of stimulation at compaction coincides with a switch in energy dependence from lactate and pyruvate to glucose and an increase in protein synthetic activity.

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