help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heldin, N. E.
Right arrow Articles by Westermark, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heldin, N. E.
Right arrow Articles by Westermark, B.

Endocrinology, Vol 122, 1042-1046, Copyright © 1988 by Endocrine Society

ARTICLES

Epidermal growth factor, but not thyrotropin, stimulates the expression of c-fos and c-myc messenger ribonucleic acid in porcine thyroid follicle cells in primary culture

NE Heldin and B Westermark
Department of Pathology, University of Uppsala, Sweden.

Previous studies have shown that TSH stimulates functional parameters in cultures of porcine follicle cells, but has no stimulatory effect on their growth. Epidermal growth factor (EGF), on the other hand, is a potent mitogen in such cells. The aim of the present investigation was to study the effect of EGF and TSH on the expression of proto-oncogene mRNA in porcine thyroid follicle cells. The addition of EGF caused a marked increase in the c-fos and c-myc mRNA levels. Stimulation of cells with 10 mU/ml TSH, however, failed to stimulate expression of the proto-oncogenes. The increase in c-fos mRNA caused by EGF was transient, with a maximum after 30 min. The c-myc transcript level increased after 30-60 min; the maximum level was at 90 min, and it decreased thereafter but remained at an elevated level throughout the 24 h of investigation. Half-maximal effects on proto-oncogene expression were obtained using 0.5-1 ng (c-fos) and 0.2-0.5 ng (c-myc) EGF/ml. Our data clearly demonstrate that increased expression of c-fos and c-myc mRNA is an early event in the prereplicative phase of EGF- stimulated porcine thyroid follicle cells. Since c-fos and c-myc expression may be necessary events in the prereplicative phase, the lack of effect of TSH on these functions may be closely related to its inability in stimulating the growth of porcine thyrocytes.


This article has been cited by other articles:


Home page
 EndocrinologyHome page
A. Franzén, E. Piek, B. Westermark, P. ten Dijke, and N.-E. Heldin
Expression of Transforming Growth Factor-{beta}1, Activin A, and Their Receptors in Thyroid Follicle Cells: Negative Regulation of Thyrocyte Growth and Function
Endocrinology, September 1, 1999; 140(9): 4300 - 4310.
[Abstract] [Full Text]

Home page
 J. Clin. Endocrinol. Metab.Home page
R. Heinrich and Z. Kraiem
The Protein Kinase A Pathway Inhibits c-jun and c-fos Protooncogene Expression Induced by the Protein Kinase C and Tyrosine Kinase Pathways in Cultured Human Thyroid Follicles
J. Clin. Endocrinol. Metab., June 1, 1997; 82(6): 1839 - 1844.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1988 by The Endocrine Society