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Endocrinology, Vol 122, 319-324, Copyright © 1988 by Endocrine Society

ARTICLES

Purification of bovine thyroid-stimulating hormone by a monoclonal antibody

AJ Lock, J van Denderen and LA Aarden
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.

A monoclonal antibody directed against bovine TSH was obtained by hybridoma technology. This antibody was specific for TSH and did not react with bovine LH and FSH. Affinity chromatography of crude TSH was performed on anti-TSH Sepharose. Bovine TSH was purified in a single step to near homogeneity by this technique, as shown by cation exchange chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified TSH. The biological activity of the hormone was not affected during the purification, as determined by [3H]thymidine incorporation of the TSH-dependent FRTL5 cell line. The results indicate that affinity purification of TSH by means of a monoclonal antibody is a simple one-step procedure for the production of biologically active, highly purified TSH.




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Copyright © 1988 by The Endocrine Society