Endocrinology, Vol 117, 2383-2391, Copyright © 1985 by Endocrine Society

ARTICLES

Corpus luteum angiogenic factor is related to fibroblast growth factor

D Gospodarowicz, J Cheng, GM Lui, A Baird, F Esch and P Bohlen

An angiogenic growth factor present in bovine corpus luteum (CL) has been purified to apparent homogeneity by a combination of differential salt precipitation, ion exchange chromatography, and heparin-Sepharose chromatography. It is a single chain polypeptide with an apparent mol wt of 15,000 and an amino acid composition similar to that previously reported for pituitary and brain fibroblast growth factor (FGF). Sequence analysis of the first 17 residues of the CL-derived growth factor identified the sequence; His-Phe-Lys-Asp-Pro-Lys-Arg-Leu-Tyr-X- Lys-Asn-Gly-Gly-X-Phe-Leu. This sequence is identical to residues 16-33 of bovine pituitary and brain FGF, indicating that the CL-derived growth factor is an amino-terminally truncated form of FGF and is otherwise similar, if not identical, to FGF. The biological activity of CL FGF is indistinguishable from that of pituitary or brain FGF. It is highly active in triggering the proliferation of cultured bovine vascular endothelial cells derived either from large vessels (aortic arch) or from corpus luteum and adrenal cortex capillaries (half- maximal stimulation at 20-40 pg/ml and saturation at 400-600 pg/ml). In vivo implants containing 50 ng to 1 microgram CL-derived growth factor stimulate neovascularization in the chorioallantoic membrane of the chick embryo. In addition to being mitogenic for vascular endothelial cells, CL FGF also stimulates the proliferation of a wide variety of mesoderm- and neuroectoderm-derived cells, including vascular smooth muscle cells, granulosa and adrenal cortex cells, rabbit costal chondrocytes, and corneal endothelial cells.

This article has been cited by other articles:

				C. Stocco, C. Telleria, and G. Gibori The Molecular Control of Corpus Luteum Formation, Function, and Regression Endocr. Rev., February 1, 2007; 28(1): 117 - 149. [Abstract] [Full Text] [PDF]

				S. von Otte, J. R. J. Paletta, S. Becker, S. Konig, M. Fobker, R. R. Greb, L. Kiesel, G. Assmann, K. Diedrich, and J.-R. Nofer Follicular Fluid High Density Lipoprotein-associated Sphingosine 1-Phosphate Is a Novel Mediator of Ovarian Angiogenesis J. Biol. Chem., March 3, 2006; 281(9): 5398 - 5405. [Abstract] [Full Text] [PDF]

				B. Berisha, M. Steffl, W. Amselgruber, and D. Schams Changes in fibroblast growth factor 2 and its receptors in bovine follicles before and after GnRH application and after ovulation Reproduction, February 1, 2006; 131(2): 319 - 329. [Abstract] [Full Text] [PDF]

				T. Sakurai, K. Tamura, S. Okamoto, T. Hara, and H. Kogo Possible Role of Cyclooxygenase II in the Acquisition of Ovarian Luteal Function in Rodents Biol Reprod, September 1, 2003; 69(3): 835 - 842. [Abstract] [Full Text] [PDF]

				T. Shimizu, J.-Y. Jiang, H. Sasada, and E. Sato Changes of Messenger RNA Expression of Angiogenic Factors and Related Receptors During Follicular Development in Gilts Biol Reprod, December 1, 2002; 67(6): 1846 - 1852. [Abstract] [Full Text] [PDF]

				I. R. Asplin, S. M. Wu, S. Mathew, G. Bhattacharjee, and S. V. Pizzo Differential regulation of the fibroblast growth factor (FGF) family by {alpha}2-macroglobulin: evidence for selective modulation of FGF-2-induced angiogenesis Blood, June 1, 2001; 97(11): 3450 - 3457. [Abstract] [Full Text] [PDF]

				M A Hull, J L Brough, D G Powe, G I Carter, D Jenkins, and C J Hawkey Expression of basic fibroblast growth factor in intact and ulcerated human gastric mucosa Gut, October 1, 1998; 43(4): 525 - 536. [Abstract] [Full Text] [PDF]

				C. M. Telleria, L. Zhong, S. Deb, R. K. Srivastava, K. S. Park, N. Sugino, O.-K. Park-Sarge, and G. Gibori Differential Expression of the Estrogen Receptors {alpha} and {beta} in the Rat Corpus Luteum of Pregnancy: Regulation by Prolactin and Placental Lactogens Endocrinology, May 1, 1998; 139(5): 2432 - 2442. [Abstract] [Full Text] [PDF]

				S. Mesiano and R. B. Jaffe Developmental and Functional Biology of the Primate Fetal Adrenal Cortex Endocr. Rev., June 1, 1997; 18(3): 378 - 403. [Abstract] [Full Text]