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Endocrinology, Vol 115, 2379-2383, Copyright © 1984 by Endocrine Society

ARTICLES

Reversible denaturation of the estrogen receptor and estimation of polypeptide chain molecular weight

D Sakai and J Gorski

The estrogen receptor protein loses its ability to bind to estrogens upon denaturation with sodium dodecyl sulfate and 2-mercaptoethanol. Binding activity is recovered at 60-80% efficiency upon removal of the denaturants, equilibration with 6 M guanidine hydrochloride, and dilution into buffer containing estrogen. Renatured receptor is similar to native receptor in affinity for 17 beta-estradiol and ability to bind DNA. Detection of receptor activity after sodium dodecyl sulfate- polyacrylamide gel electrophoresis of uterine or pituitary cytosol or of uterine nuclear extracts reveals a single unique polypeptide species of 65,000 mol wt.


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G. Greene, P Gilna, M Waterfield, A Baker, Y Hort, and J Shine
Sequence and expression of human estrogen receptor complementary DNA
Science, March 7, 1986; 231(4742): 1150 - 1154.
[Abstract] [PDF]


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