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Endocrinology, Vol 114, 1124-1127, Copyright © 1984 by Endocrine Society

ARTICLES

Ultrastructural localization of relaxin immunoreactivity in corpora lutea of pregnant rats

MB Anderson and OD Sherwood

Relaxin was localized in cells of corpora lutea of pregnant rats at the ultrastructural level using a highly specific antirat relaxin serum and the unlabeled peroxidase-antiperoxidase technique. Electron-dense, membrane-bound granules (maximum diameter, 270 nm), which are present in luteal cells during the last third of gestation, were the only inclusions that were immunochemically stained. The number of granules observed in the luteal cell cytoplasm varied from cell to cell within a particular section. Furthermore, in the granule-rich luteal cells, the granules appeared in clusters. This study establishes that these electron-dense granules represent the subcellular sites of relaxin localization within luteal cells of pregnant rats.


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[Abstract] [Full Text]


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