help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Evans, R. W.
Right arrow Articles by Braverman, L. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Evans, R. W.
Right arrow Articles by Braverman, L. E.

Endocrinology, Vol 113, 1459-1463, Copyright © 1983 by Endocrine Society

ARTICLES

Nuclear thyroid hormone receptor in the rat uterus

RW Evans, AP Farwell and LE Braverman

The uterus may be a target organ for T3 action. The present study was done to determine whether uterine nuclei contain receptors for T3 (T3R). Methods were established for the measurement of T3R concentrations in preparations of rat uterine nuclei. The liver was studied concomitantly as a tissue known to contain nuclear T3R. Nuclear fractions were prepared by established procedures and extracted in buffer containing 0.5 M KCl. Specific [125I]T3 binding in the nuclear extract was analyzed by Scatchard plot. In both liver and uterine nuclear extracts, a binding component of high affinity for [125I]T3 (Ka = 1.0-3.0 X 10(9) M-1) was detected. This binding component was lost after heating at 50 C for 10 min. On sucrose density gradients, the principal [125I]T3-binding component in uterine and liver nuclear extracts had a 3.5-4.0 S sedimentation coefficient. Binding specificity was assessed by competition assays for [125I]T3 binding by using stable T3, T4, triiodothyroacetic acid (TRIAC), rT3, and 3,5-diiodothyronine (T2). The order of relative binding affinities (RBAs) in uterine nuclear extracts was T3 greater than TRIAC greater than T4 greater than rT3 greater than T2, and that in liver extracts was TRIAC greater than T3 greater than T4 greater than rT3 greater than T2. In contrast, RBA values derived from dilute serum were distinctly different from both uterine and liver values: T4 greater than T3 greater than rT3 greater than TRIAC greater than T2. The concentrations (femtomoles per mg DNA) of T3R in several tissues were: liver, 595 +/- 162; kidney, 492 +/- 120; uterus, 249 +/- 66; spleen, 48 +/- 10 (mean +/- SE; n = 4). These results provide evidence for the presence of T3R in the nuclear fraction of the rat uterus with properties similar to those of the liver nuclear T3R.


This article has been cited by other articles:


Home page
 EndocrinologyHome page
V. A. Galton, E. Martinez, A. Hernandez, E. A. St. Germain, J. M. Bates, and D. L. St. Germain
The Type 2 Iodothyronine Deiodinase Is Expressed in the Rat Uterus and Induced During Pregnancy
Endocrinology, May 1, 2001; 142(5): 2123 - 2128.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1983 by The Endocrine Society