Endocrinology, Vol 112, 1156-1158, Copyright © 1983 by Endocrine Society

ARTICLES

Regulation of cytochrome P-450scc in immature porcine granulosa cells by FSH and estradiol

ME Toaff, JF Strauss 3d and JM Hammond

FSH, estradiol, or a combination of FSH and estradiol enhanced secretion of progesterone by primary cultures of immature porcine granulosa cells; they also increased the mitochondrial content of cytochrome P-450 and mitochondrial cholesterol side-chain cleavage activity. FSH or estradiol alone increased mitochondrial cytochrome P- 450 levels and cholesterol side-chain cleavage activity to a similar extent. The combination of FSH and estradiol further increased cytochrome P-450 and side-chain cleavage activity. Our results demonstrate that FSH, estrogen and the combination of the hormones stimulate progestin synthesis by granulosa cells, at least in part, by increasing cholesterol side-chain cleavage enzyme levels. These observations suggest a dual hormonal regulation of the enzyme catalyzing the rate-limiting step in the biosynthetic pathway of steroid hormones.

This article has been cited by other articles:

				E. P. Fiedler, L. Jr. Plouffe, D. B. Hales, K. H. Hales, and I. Khan Prostaglandin F2{alpha} Induces a Rapid Decline in Progesterone Production and Steroidogenic Acute Regulatory Protein Expression in Isolated Rat Corpus Luteum Without Altering Messenger Ribonucleic Acid Expression Biol Reprod, September 1, 1999; 61(3): 643 - 650. [Abstract] [Full Text]

				J. S. Babischkin, G. J. Pepe, and E. D. Albrecht Estrogen Regulation of Placental P-450 Cholesterol Side-Chain Cleavage Enzyme Messenger Ribonucleic Acid Levels and Activity During Baboon Pregnancy Endocrinology, January 1, 1997; 138(1): 452 - 459. [Abstract] [Full Text] [PDF]