Endocrinology, Vol 112, 1153-1155, Copyright © 1983 by Endocrine Society

ARTICLES

Thyroxine 5'-deiodinase activity in brown adipose tissue

JL Leonard, SA Mellen and PR Larsen

Homogenates of rat brown adipose tissue (BAT) and epididymal fat pad (EPI) catalyze the 5'-deiodination of 3,3',5'-triiodothyronine (rT3) in the presence of dithiothreitol. BAT, but not EPI, catalyzes the 5'- deiodination of thyroxinE (T4) generating equal amounts of 3,5,3'- triiodothyronine (T3) and iodide. BAT 5'-deiodinase activity was unaffected by propylthiouracil (PTU) and was elevated in hypothyroid rats, whereas EPI 5'-deiodinase was totally inhibited by PTU and was depressed in hypothyroidism. These results indicate that type II (PTU- insensitive) 5'-iodothyronine deiodinase activity is present in BAT.

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