help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Raymoure, W. J.
Right arrow Articles by Kuhn, R. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Raymoure, W. J.
Right arrow Articles by Kuhn, R. W.

Endocrinology, Vol 112, 1091-1097, Copyright © 1983 by Endocrine Society

ARTICLES

A homologous radioimmunoassay for rat corticosteroid-binding globulin

WJ Raymoure and RW Kuhn

A rapid, specific, and sensitive RIA was developed for rat corticosteroid-binding globulin (CBG). Rat CBG was purified by affinity chromatography and its precise concentration was determined by amino acid analysis. This rat CBG was injected into rabbits to raise antiserum and was used both as the assay standard and as the tracer after labeling with 125I. Antiserum to CBG was judged specific by immunoelectrophoresis and by the comparison of RIA values with steroid- binding assay values obtained following serum fractionation on ion exchange and sizing resins. The RIA was used to determine CBG levels in pregnant rats (2.65 microM on day 14 falling to 0.95 microM at parturition), their corresponding fetuses (0.24 microM on day 18 and 0.16 microM at parturition), and amniotic fluid (0.051 microM on day 13 rising to 0.21 microM on day 21).




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1983 by The Endocrine Society